Quantitative analysis of the profiles of twelve major compounds in Gentiana straminea Maxim. Roots by LC-MS/MS in an extensive germplasm survey in the Qinghai-Tibetan plateau

NWIPB OpenIR

	Quantitative analysis of the profiles of twelve major compounds in Gentiana straminea Maxim. Roots by LC-MS/MS in an extensive germplasm survey in the Qinghai-Tibetan plateau
	Zhou, Dangwei; Lv, Dongjin; Zhang, Hui; Cheng, Tingfeng; Wang, Huan; Lin, Pengcheng; Shi, Shengbo; Chen, Shilong; Shen, Jianwei
	2021
发表期刊	JOURNAL OF ETHNOPHARMACOLOGY
卷号	280
摘要	Ethnobotanical relevance: Gentiana straminea Maxim. is a well-known Tibetan traditional herb, which has been used to treat rheumatic arthritis, iceteric hepatitis, and other diseases for thousands years. However, there is still lack of comprehensive active constituents profiling of this species throughout the Qinghai-Tibet Plateau (QTP). Aim of study: This study was designed to provide a comprehensive quality map of G.straminea germplasm based on twelve active constituents (loganic acid, gentiopicroside, swertiamarin, sweroside, 6-O-beta-D-glucosylgentio-picroside, oleanic acid, morroniside, trilobatin, isoorientin, isovite, Shanzhisidemethylester and quercetin) on the QTP. Materials and methods: G.straminea root samples collected throughout QTP in the flowering period were analyzed by the LC-MS/MS. Statistics analysis methods PCA, clustering and ecological regions analysis for G.straminea constituents differentiation was demonstrated. Results: The active constituents varied greatly across the QTP; the majority of constituents were secoiridoid derivatives, with gentiopicroside being the most abundant compound. Most constituents were significantly affected by the latitudes and altitudes other than longitudes. PCA and hierarchical clustering analysis showed that all samples could be separated into six distinct groups, and 15 populations showed the highest constituent abundances. Further, geographical region analysis showed that the highest quality populations mainly located near the source region of Yellow River, especially in the Qinghai and Sichuan areas. Additionally, correlation analysis showed that there were relationships among genetiopicroside, loganic acid, and other compounds, which might be related to the enzymatic pathways involved in the metabolism of these constituents. Conclusion: LC-MS/MS method allowed separation of quality profiling of G.straminea on the QTP, 15 populations showed the highest constituent abundances. In six geographical groups, the highest quality populations mainly located near the source region of Yellow River, especially in the Qinghai and Sichuan areas, which may be due to the climate caused by the westerlies and Indian Ocean monsoons in the QTP.
关键词	Gentiana straminea Maxim Active compounds LC-MS MS Qinghai-Tibetan plateau Source region of yellow river
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60670
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhou, Dangwei,Lv, Dongjin,Zhang, Hui,et al. Quantitative analysis of the profiles of twelve major compounds in Gentiana straminea Maxim. Roots by LC-MS/MS in an extensive germplasm survey in the Qinghai-Tibetan plateau[J]. JOURNAL OF ETHNOPHARMACOLOGY,2021,280.
APA	Zhou, Dangwei.,Lv, Dongjin.,Zhang, Hui.,Cheng, Tingfeng.,Wang, Huan.,...&Shen, Jianwei.(2021).Quantitative analysis of the profiles of twelve major compounds in Gentiana straminea Maxim. Roots by LC-MS/MS in an extensive germplasm survey in the Qinghai-Tibetan plateau.JOURNAL OF ETHNOPHARMACOLOGY,280.
MLA	Zhou, Dangwei,et al."Quantitative analysis of the profiles of twelve major compounds in Gentiana straminea Maxim. Roots by LC-MS/MS in an extensive germplasm survey in the Qinghai-Tibetan plateau".JOURNAL OF ETHNOPHARMACOLOGY 280(2021).