Visualizing the hypoxic heterogeneity for distinguishing the cancer tissues with a two-photon nitroreductase-H2S logic probe via intramolecular isomerization

NWIPB OpenIR

	Visualizing the hypoxic heterogeneity for distinguishing the cancer tissues with a two-photon nitroreductase-H2S logic probe via intramolecular isomerization
	Xue, Junlian; Liu, Yuxia; Li, Lu; Xu, Jie; Chen, Tao; Li, Yulin; Chen, Guang
	2021
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
卷号	347
摘要	The uncontrollable growth of tumor leads to the heterogeneity of hypoxia, which is more complicated than researchers have detected using a single factor. Using two factors to reveal the heterogeneous hypoxic environment would be more accurate for distinguishing the cancerous tissues. Herein, we develop a two-photon fluorescence probe (E)-1-(2-(2,4-dinitrophenoxy)naphthalen-1-yl)-2-(4-nitrophenyl)diazene (DNHF) featuring the logic responses toward two factors nitroreductase (NTR) and H2S via intramolecular isomerization, for the imaging of the hypoxic heterogeneity. DNHF is transformed respectively by H2S or NTR to a hydrazone structure (DND) or a reduced product DAHF. Interestingly, DND and DAHF is almost quenched due to the interinhibitive effect or the hydrazone isomerization. While the product AHF triggered by the coexistent high level of NTR and H2S shows strong fluorescence via an intermolecular isomerization to the azo-structure AHF-2. These properties enable DNHF to reveal the severe hypoxia (high NTR and H2S) by releasing the strong fluorescence and the mild hypoxia (low NTR or H2S) by releasing the weak fluorescence, thereby visualizing the hypoxic heterogeneity by two factors. With the DNHF, the heterogeneity difference between normal and tumor tissues is enhanced. We envision this probe may serve as an effective tool for the accurate analysis of cancer tissues.
关键词	Nitroreductase-H2S logic probe Hypoxic heterogeneity Intramolecular isomerization Tumor tissue
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60671
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xue, Junlian,Liu, Yuxia,Li, Lu,et al. Visualizing the hypoxic heterogeneity for distinguishing the cancer tissues with a two-photon nitroreductase-H2S logic probe via intramolecular isomerization[J]. SENSORS AND ACTUATORS B-CHEMICAL,2021,347.
APA	Xue, Junlian.,Liu, Yuxia.,Li, Lu.,Xu, Jie.,Chen, Tao.,...&Chen, Guang.(2021).Visualizing the hypoxic heterogeneity for distinguishing the cancer tissues with a two-photon nitroreductase-H2S logic probe via intramolecular isomerization.SENSORS AND ACTUATORS B-CHEMICAL,347.
MLA	Xue, Junlian,et al."Visualizing the hypoxic heterogeneity for distinguishing the cancer tissues with a two-photon nitroreductase-H2S logic probe via intramolecular isomerization".SENSORS AND ACTUATORS B-CHEMICAL 347(2021).