Lateral flow immunoassay for furazolidone point-of-care testing: Cater to the call of saving time, labor, and cost by coomassie brilliant blue labeling

NWIPB OpenIR

	Lateral flow immunoassay for furazolidone point-of-care testing: Cater to the call of saving time, labor, and cost by coomassie brilliant blue labeling
	Xu, Jingke; Dou, Leina; Liu, Sijie; Su, Lihong; Yin, Xuechi; Ren, Jing; Hu, Huilan; Zhang, Daohong; Sun, Jing; Wang, Zhanhui; Wang, Jianlong
	2021
发表期刊	FOOD CHEMISTRY
卷号	352
摘要	Furazolidone (FZD) and its metabolite called 3-amino-2-oxazolidinone (AOZ) would induce carcinogenic and mutagenic effects to human. In this work, to develop a novel, stable, and simple point of care testing (POCT) with a potential to social applied for FZD detection, we utilized the aspect of protein staining of coomassie brilliant blue (CBB) to exploit a new CBB-LFIA strategy free of NPs. Only one mixing step is needed during the probe manufacturing process, which requires just 2 h and is a great time saving strategy compared with other methods (requiring 4?33 h for probe preparation). Besides, the cost of CBB-LFIA is 300 times lesser than other LFIA with respect to obtaining the label. The developed CBB-LFIA was successfully applied to detect AOZ with a detection limit of 2 ng mL-1, without any influence from other potential interfering compounds. The proposed CBB-LFIA exhibited prominent practical application, and possesses considerable utilization potential in the related field.
关键词	Lateral flow immunoassay Point-of-care testing Coomassie brilliant blue Furazolidone detection
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60763
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xu, Jingke,Dou, Leina,Liu, Sijie,et al. Lateral flow immunoassay for furazolidone point-of-care testing: Cater to the call of saving time, labor, and cost by coomassie brilliant blue labeling[J]. FOOD CHEMISTRY,2021,352.
APA	Xu, Jingke.,Dou, Leina.,Liu, Sijie.,Su, Lihong.,Yin, Xuechi.,...&Wang, Jianlong.(2021).Lateral flow immunoassay for furazolidone point-of-care testing: Cater to the call of saving time, labor, and cost by coomassie brilliant blue labeling.FOOD CHEMISTRY,352.
MLA	Xu, Jingke,et al."Lateral flow immunoassay for furazolidone point-of-care testing: Cater to the call of saving time, labor, and cost by coomassie brilliant blue labeling".FOOD CHEMISTRY 352(2021).