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Protective Effect of Two Alkaloids from Hippophae rhamnoides Linn. against Doxorubicin-Induced Toxicity in H9c2 Cardiomyoblasts | |
Zhou, Wenna; Ouyang, Jian; Hu, Na![]() ![]() | |
2021 | |
发表期刊 | MOLECULES
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卷号 | 26期号:7 |
摘要 | Background: Doxorubicin (Dox) is one of the most frequently prescribed anti-cancer drugs. However, clinical application with Dox is limited due to its potentially fatal cumulative cardiotoxicity. N-p-coumaroyl-4-aminobutan-1-ol (alk-A), an organic amide alkaloid and hippophamide (alk-B), a rare pyridoindole alkaloid were successfully obtained by purification and separation of seabuckthorn seed residue in our previous research. This study was undertaken to investigate the protective effect of alk-A and alk-B against Dox-induced embryonic rat cardiac cells (H9c2 cells) apoptosis. Methods: H9c2 cells were treated with Dox (2.5 mu M) in the presence of alk-A and alk-B (10, 20, and 40 mu M) and incubated for 24 h. Results: It was shown that pretreatment of the H9c2 cells with alk-A and alk-B significantly reduced Dox-induced apoptosis. Alk-A and alk-B both inhibited reactive oxygen species (ROS) production and suppressed cleaved-caspase-3 protein expression and the activation of JNK (Jun N-terminal kinases), as well as increasing ATP levels, favoring mitochondrial mitofusin protein expression, and relieving damage to mitochondrial DNA. Conclusions: These results suggest that alk-A and alk-B can inhibit Dox-induced apoptosis in H9C2 cardiac muscle cells via inhibition of cell apoptosis and improvement of mitochondrial function, while alk-B showed more protection. Alk-B could be a potential candidate agent for protecting against cardiotoxicity in Dox-exposed patients. |
关键词 | alkaloid doxorubicin apoptosis mitochondrial function seabuckthorn |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/60824 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Zhou, Wenna,Ouyang, Jian,Hu, Na,et al. Protective Effect of Two Alkaloids from Hippophae rhamnoides Linn. against Doxorubicin-Induced Toxicity in H9c2 Cardiomyoblasts[J]. MOLECULES,2021,26(7). |
APA | Zhou, Wenna,Ouyang, Jian,Hu, Na,Li, Gang,&Wang, Honglun.(2021).Protective Effect of Two Alkaloids from Hippophae rhamnoides Linn. against Doxorubicin-Induced Toxicity in H9c2 Cardiomyoblasts.MOLECULES,26(7). |
MLA | Zhou, Wenna,et al."Protective Effect of Two Alkaloids from Hippophae rhamnoides Linn. against Doxorubicin-Induced Toxicity in H9c2 Cardiomyoblasts".MOLECULES 26.7(2021). |
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