Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Bioresource-derived tannic acid-supported immuno-network in lateral flow immunoassay for sensitive clenbuterol monitoring | |
Liu, SJ; Shu, R; Nie, C; Li, YC; Luo, X; Ji, YW; Yin, XC; Sun, J; Zhang, DH; Wang, JL | |
2022 | |
发表期刊 | FOOD CHEMISTRY |
卷号 | 382 |
摘要 | Although lateral flow immunoassay (LFIA) as a point-of-care (POC) diagnostic tool has been widely used because of numerous merits, it remains challenging to realize higher sensitivity, easier labeling, and fewer antibodies consumption. Herein, a bioresource-derived tannic acid (TA)-supported immuno-network based LFIA for clenbuterol (CLE) monitoring was proposed by employing poly TA nanospheres (PTAN) as the new tracer. Attributing to the effective protein-enrichment ability of TA, plenty of goat anti-mouse immunoglobulins (GAMI) were first absorbed on the surface of PTAN and then monoclonal antibodies (Ab(CLE)) were added to form immuno-network. Compared with directly coupling antibodies, the utilization efficiency of Ab(CLE) was significantly improved. Thus, the detection sensitivity (take the cut-off value as an example) was enhanced 5-fold and 10-fold at least compared with PTAN-Ab(CLE) and colloidal gold-based LFIA, respectively. Additionally, the proposed method was successfully verified in beef and pork liver and provided an effective strategy for food safety monitoring. |
收录类别 | SCIE |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/61154 |
专题 | 中国科学院西北高原生物研究所 |
推荐引用方式 GB/T 7714 | Liu, SJ,Shu, R,Nie, C,et al. Bioresource-derived tannic acid-supported immuno-network in lateral flow immunoassay for sensitive clenbuterol monitoring[J]. FOOD CHEMISTRY,2022,382. |
APA | Liu, SJ.,Shu, R.,Nie, C.,Li, YC.,Luo, X.,...&Wang, JL.(2022).Bioresource-derived tannic acid-supported immuno-network in lateral flow immunoassay for sensitive clenbuterol monitoring.FOOD CHEMISTRY,382. |
MLA | Liu, SJ,et al."Bioresource-derived tannic acid-supported immuno-network in lateral flow immunoassay for sensitive clenbuterol monitoring".FOOD CHEMISTRY 382(2022). |
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