Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect | |
Lu, Shuaimin1; Li, Guoliang1,2; Lv, Zhengxian1; Qiu, Nannan2; Kong, Weiheng1; Gong, Peiwei1; Chen, Guang1; Xia, Lian1; Guo, Xiaoxi1; You, Jinmao1,3; Wu, Yongning2 | |
2016-11-15 | |
发表期刊 | BIOSENSORS & BIOELECTRONICS |
卷号 | 85页码:358-362 |
文章类型 | Article |
摘要 | Early detection and diagnosis have great practical significances for the effective prevention and treatment of cancer. In this study, we developed a novel, facile and ultra-sensitive fluorescence assay for the determination of tumor invasive biomarker beta-glucuronidase (GLU) based on the inner-filter effect (IFE). The nitrogen-doped carbon quantum dots (N-CQDs) with green photoluminescence were employed as the fluorophore in IFE, and 4-nitrophenyl-beta-D-glucuronide (PNPG) was used to act as GLU substrate, and GLU catalytic product (p-nitrophenol (PNP)) was capable of acting as the robust absorber in IFE to turn off the fluorescence of N-CQDs due to the complementary overlap between the absorption of PNP and the excitation of N-CQDs. Thus, signal of GLU activity could be recorded by the fluorescence intensity of N-CQDs. Unlike other fluorescence sensing mechanism such as fluorescence resonance energy transfer (FRET) or photoinduced electron transfer (PET), IFE has no requirement for electron or energy transfer process or any chemical modification of fluorophore, which makes our assay more flexible and simple. The proposed method exhibited a good linear relationship from 1 U L-1 to 60 U L-1 (R-2=0.9967) with a low detection limit of 0.3 U L-1. This method was also successfully applied to the analysis of serum samples and the inhibitor screening from natural product. The developed sensor platform was proven to be reliable, facile, sensitive, and selective, making it promising as a candidate for GLU activity detection in clinic tumor diagnose and anti-tumor drug screening. (C) 2016 Elsevier B.V. All rights reserved. |
关键词 | Beta-glucuronidase Nitrogen-doped Carbon Quantum Dots Inner-filter Effect Anti-tumor Drug Screening |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1016/j.bios.2016.05.021 |
关键词[WOS] | COLON-CANCER ; NATURAL-PRODUCTS ; OPTIMIZATION ; PROBE |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | China Postdoctoral Science Foundation(2016M590071) ; National Natural Science Foundation of China(31301595 ; Natural Science Foundation of Shandong Province, China(ZR2013BQ019) ; Experimental technology research projects of QFNU(SJ201505) ; Qufu Normal University(bsqd 2012017) ; 21475074 ; 21475075 ; 21275089) |
WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
WOS记录号 | WOS:000382410100048 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6444 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.China Natl Ctr Food Safety Risk Assessment, Key Labs Chem Safety & Hlth, Beijing 100050, Peoples R China 3.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Peoples R China |
推荐引用方式 GB/T 7714 | Lu, Shuaimin,Li, Guoliang,Lv, Zhengxian,et al. Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect[J]. BIOSENSORS & BIOELECTRONICS,2016,85:358-362. |
APA | Lu, Shuaimin.,Li, Guoliang.,Lv, Zhengxian.,Qiu, Nannan.,Kong, Weiheng.,...&Wu, Yongning.(2016).Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect.BIOSENSORS & BIOELECTRONICS,85,358-362. |
MLA | Lu, Shuaimin,et al."Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect".BIOSENSORS & BIOELECTRONICS 85(2016):358-362. |
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