NWIPB OpenIR
Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect
Lu, Shuaimin1; Li, Guoliang1,2; Lv, Zhengxian1; Qiu, Nannan2; Kong, Weiheng1; Gong, Peiwei1; Chen, Guang1; Xia, Lian1; Guo, Xiaoxi1; You, Jinmao1,3; Wu, Yongning2
2016-11-15
Source PublicationBIOSENSORS & BIOELECTRONICS
Volume85Pages:358-362
SubtypeArticle
AbstractEarly detection and diagnosis have great practical significances for the effective prevention and treatment of cancer. In this study, we developed a novel, facile and ultra-sensitive fluorescence assay for the determination of tumor invasive biomarker beta-glucuronidase (GLU) based on the inner-filter effect (IFE). The nitrogen-doped carbon quantum dots (N-CQDs) with green photoluminescence were employed as the fluorophore in IFE, and 4-nitrophenyl-beta-D-glucuronide (PNPG) was used to act as GLU substrate, and GLU catalytic product (p-nitrophenol (PNP)) was capable of acting as the robust absorber in IFE to turn off the fluorescence of N-CQDs due to the complementary overlap between the absorption of PNP and the excitation of N-CQDs. Thus, signal of GLU activity could be recorded by the fluorescence intensity of N-CQDs. Unlike other fluorescence sensing mechanism such as fluorescence resonance energy transfer (FRET) or photoinduced electron transfer (PET), IFE has no requirement for electron or energy transfer process or any chemical modification of fluorophore, which makes our assay more flexible and simple. The proposed method exhibited a good linear relationship from 1 U L-1 to 60 U L-1 (R-2=0.9967) with a low detection limit of 0.3 U L-1. This method was also successfully applied to the analysis of serum samples and the inhibitor screening from natural product. The developed sensor platform was proven to be reliable, facile, sensitive, and selective, making it promising as a candidate for GLU activity detection in clinic tumor diagnose and anti-tumor drug screening. (C) 2016 Elsevier B.V. All rights reserved.
KeywordBeta-glucuronidase Nitrogen-doped Carbon Quantum Dots Inner-filter Effect Anti-tumor Drug Screening
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.bios.2016.05.021
WOS KeywordCOLON-CANCER ; NATURAL-PRODUCTS ; OPTIMIZATION ; PROBE
Indexed BySCI
Language英语
Funding OrganizationChina Postdoctoral Science Foundation(2016M590071) ; National Natural Science Foundation of China(31301595 ; Natural Science Foundation of Shandong Province, China(ZR2013BQ019) ; Experimental technology research projects of QFNU(SJ201505) ; Qufu Normal University(bsqd 2012017) ; 21475074 ; 21475075 ; 21275089)
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000382410100048
Citation statistics
Cited Times:46[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/6444
Collection中国科学院西北高原生物研究所
Affiliation1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China
2.China Natl Ctr Food Safety Risk Assessment, Key Labs Chem Safety & Hlth, Beijing 100050, Peoples R China
3.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining 810001, Peoples R China
Recommended Citation
GB/T 7714
Lu, Shuaimin,Li, Guoliang,Lv, Zhengxian,et al. Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect[J]. BIOSENSORS & BIOELECTRONICS,2016,85:358-362.
APA Lu, Shuaimin.,Li, Guoliang.,Lv, Zhengxian.,Qiu, Nannan.,Kong, Weiheng.,...&Wu, Yongning.(2016).Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect.BIOSENSORS & BIOELECTRONICS,85,358-362.
MLA Lu, Shuaimin,et al."Facile and ultrasensitive fluorescence sensor platform for tumor invasive biomaker beta-glucuronidase detection and inhibitor evaluation with carbon quantum dots based on inner-filter effect".BIOSENSORS & BIOELECTRONICS 85(2016):358-362.
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