Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecum L.) by HSCCC

doi:10.1093/chromsci/bmv169

NWIPB OpenIR

	Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecum L.) by HSCCC
	He, Yanfeng 1,2,3; Wang, Xiaoyan 1; Suo, Yourui 1; Ding, Chenxu 1; Wang, Honglun 1
	2016-03-01
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷号	54 期号:3 页码:479-485
文章类型	Article
摘要	High efficiency and less solvent consumption are the essential requirements of high-speed countercurrent chromatography (HSCCC), especially for the large-scale preparation. In this study, an efficient HSCCC strategy with consecutive sample injection was successfully developed to rapidly separate and purify rhaponticin and rhapontigenin from the seeds of the Chinese medicinal herb fenugreek (Trigonella foenum-graecum L.). The effective separation was achieved using n-hexane-ethyl acetate-methanol-water (1: 4: 2: 6, v/v/v/v) as the two-phase solvent system, in which the mobile phase was eluted at an optimized flow rate of 2.2 mL/min and a revolution speed of 850 rpm. After consecutively loading four identical fenugreek samples, each containing 120 mg, HSCCC separation yielded 146.4 mg of rhaponticin and 174.8 mg of rhapontigenin with purities of 98.6 and 99.1%, respectively, as determined by high-performance liquid chromatography at 320 nm. Their chemical structures were identified using UV spectroscopy, H-1-NMR and C-13-NMR. The HSCCC method with consecutive sample injection allowed faster separation and produced less solvent waste, suggesting that it is an efficient way to rapidly separate and purify natural products on a large scale.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1093/chromsci/bmv169
关键词[WOS]	COUNTER-CURRENT CHROMATOGRAPHY ; PREPARATIVE ISOLATION ; ANTIOXIDANT ACTIVITIES ; PURIFICATION ; SEPARATION ; SEEDS ; LIVER ; FLAVONOIDS ; CAPACITY ; PLASMA
收录类别	SCI
语种	英语
项目资助者	Natural Science Foundation of China(81001698 ; Funds for Outstanding Youth in Research Field of Life Science, Chinese Academy of Sciences, China(KSCX2-EW-Q-23) ; International Cooperation Program of Qinghai Province(2013-H-802) ; 31300292 ; 31470426)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000380263300026
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6461
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining, Peoples R China 2.Qinghai Nationalities Univ, Coll Pharm, Xining, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	He, Yanfeng,Wang, Xiaoyan,Suo, Yourui,et al. Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecum L.) by HSCCC[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016,54(3):479-485.
APA	He, Yanfeng,Wang, Xiaoyan,Suo, Yourui,Ding, Chenxu,&Wang, Honglun.(2016).Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecum L.) by HSCCC.JOURNAL OF CHROMATOGRAPHIC SCIENCE,54(3),479-485.
MLA	He, Yanfeng,et al."Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecum L.) by HSCCC".JOURNAL OF CHROMATOGRAPHIC SCIENCE 54.3(2016):479-485.