Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecum L.) by HSCCC | |
He, Yanfeng1,2,3; Wang, Xiaoyan1; Suo, Yourui1; Ding, Chenxu1; Wang, Honglun1 | |
2016-03-01 | |
发表期刊 | JOURNAL OF CHROMATOGRAPHIC SCIENCE
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卷号 | 54期号:3页码:479-485 |
文章类型 | Article |
摘要 | High efficiency and less solvent consumption are the essential requirements of high-speed countercurrent chromatography (HSCCC), especially for the large-scale preparation. In this study, an efficient HSCCC strategy with consecutive sample injection was successfully developed to rapidly separate and purify rhaponticin and rhapontigenin from the seeds of the Chinese medicinal herb fenugreek (Trigonella foenum-graecum L.). The effective separation was achieved using n-hexane-ethyl acetate-methanol-water (1: 4: 2: 6, v/v/v/v) as the two-phase solvent system, in which the mobile phase was eluted at an optimized flow rate of 2.2 mL/min and a revolution speed of 850 rpm. After consecutively loading four identical fenugreek samples, each containing 120 mg, HSCCC separation yielded 146.4 mg of rhaponticin and 174.8 mg of rhapontigenin with purities of 98.6 and 99.1%, respectively, as determined by high-performance liquid chromatography at 320 nm. Their chemical structures were identified using UV spectroscopy, H-1-NMR and C-13-NMR. The HSCCC method with consecutive sample injection allowed faster separation and produced less solvent waste, suggesting that it is an efficient way to rapidly separate and purify natural products on a large scale. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1093/chromsci/bmv169 |
关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; PREPARATIVE ISOLATION ; ANTIOXIDANT ACTIVITIES ; PURIFICATION ; SEPARATION ; SEEDS ; LIVER ; FLAVONOIDS ; CAPACITY ; PLASMA |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Natural Science Foundation of China(81001698 ; Funds for Outstanding Youth in Research Field of Life Science, Chinese Academy of Sciences, China(KSCX2-EW-Q-23) ; International Cooperation Program of Qinghai Province(2013-H-802) ; 31300292 ; 31470426) |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS记录号 | WOS:000380263300026 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6461 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining, Peoples R China 2.Qinghai Nationalities Univ, Coll Pharm, Xining, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | He, Yanfeng,Wang, Xiaoyan,Suo, Yourui,et al. Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecum L.) by HSCCC[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016,54(3):479-485. |
APA | He, Yanfeng,Wang, Xiaoyan,Suo, Yourui,Ding, Chenxu,&Wang, Honglun.(2016).Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecum L.) by HSCCC.JOURNAL OF CHROMATOGRAPHIC SCIENCE,54(3),479-485. |
MLA | He, Yanfeng,et al."Efficient Protocol for Isolation of Rhaponticin and Rhapontigenin with Consecutive Sample Injection from Fenugreek (Trigonella foenum-graecum L.) by HSCCC".JOURNAL OF CHROMATOGRAPHIC SCIENCE 54.3(2016):479-485. |
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