One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography

doi:10.1093/chromsci/bmv153

NWIPB OpenIR

	One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography
	Zhang, Qiulong 1,2; Ma, Tao 1,2; Hu, Na 1,2; Ding, Chenxi 3; Liang, Yongxin 4; Li, Wencong 1,2; Suo, Yourui 1; Ding, Chenxu 1,4,5
	2016-03-01
发表期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷号	54 期号:3 页码:466-471
文章类型	Article
摘要	High-speed counter-current chromatography (CCC) was firstly and successfully applied for the preparative separation and purification of alkaloids from crude extract of Hypecoum leptocarpum. After the measurement of partition coefficient of five target alkaloids in the two-phase solvent systems, the CCC was performed well with a two-phase solvent system composed of tetrachloromethane-chloroform-methanol-0.1 M HCl at a volume ratio of 1.5 : 2.5 : 3 : 2 (V/V/V/V). The upper phase was used as the stationary phase, and the lower phasewas used as the mobile phase. From 120 mg crude extract, 5 mg leptopidine, 32 mg oxohydrastinine, 27 mg (-)-N-methylanadine, 7 mg N-feruloyltyramine and 3 mg hypecoleptopine could be successfully separated. The amides alkaloid, N-feruloyltyramine, was firstly separated from H. leptocarpum. High-performance liquid chromatography analysis showed that the purity of each of the five target alkaloids was over 92%. Their chemical structures were confirmed by H-1-NMR and C-13-NMR data.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1093/chromsci/bmv153
关键词[WOS]	INHIBITORY-ACTIVITY ; HOOK-F ; THOMS
收录类别	SCI
语种	英语
项目资助者	Chinese Ministry of Education(NO212188) ; Bureau of Science and Technology(2014-HZ-810) ; State Key Laboratory Breeding Base (Key Laboratory for Plateau Crop Germplasm Innovation and Utilization of Qinghai Province)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000380263300024
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6465
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, 23 Xinning Rd, Xining 810008, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Qinghai Red Cross Hosp, Xining 810000, Peoples R China 4.Qinghai Nationalities Univ, Xining 810016, Peoples R China 5.Key Lab Plateau Crop Germplasm Innovat & Utilizat, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Qiulong,Ma, Tao,Hu, Na,et al. One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2016,54(3):466-471.
APA	Zhang, Qiulong.,Ma, Tao.,Hu, Na.,Ding, Chenxi.,Liang, Yongxin.,...&Ding, Chenxu.(2016).One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE,54(3),466-471.
MLA	Zhang, Qiulong,et al."One Step to Separate Five Alkaloids from Hypecoum leptocarpum by High-Speed Counter-Current Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE 54.3(2016):466-471.