Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry

doi:10.1016/j.jchromb.2015.11.015

NWIPB OpenIR

	Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry
	Zhang, Yun 1; Jin, Hongli 1; Li, Xiaolong 1; Zhao, Jianqiang 2; Guo, Xiujie 1; Wang, Jixia 1; Guo, Zhimou 1; Zhang, Xiuli 1; Tao, Yanduo 2; Liu, Yanfang 1; Chen, Deliang 3; Liang, Xinmiao 1
	2016-07-15
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
卷号	1026 页码:67-74
文章类型	Article
摘要	Bufadienolides possess various bioactivities especially antitumor. Due to the high structural diversity, the separation of bufadienolides often suffers from coelution problem on conventional RP columns. In this work, an off-line two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography (2D-NPLC x RPLC) method was developed to separate and characterize bufadienolides in toad skin. Several RP and NP columns were evaluated with five reference bufadienlides. The XUnion C18 and XAmide columns exhibited superior chromatographic performances for bufadienlide separation, and were selected in RPLC and NPLC, respectively. RPLC was used in the second-dimension for the good compatibility with MS, while NPLC was adopted in the first-dimension. The orthogonality of the 2D-NPLC x RPLC system was investigated by the geometric approach using fifteen bufadienolide mixtures. The result was 49.6%, demonstrating reasonable orthogonality of this 2D-LC system. By combining the 2D-LC system with MS, 64 bufadienlides including 33 minor ones and 11 pairs of isomers in toad skin were identified. This off-line 2D-NPLC x RPLC allowed to solve the coelution problem of bufadienlides in one-dimension RPLC, and thus facilitated the identification significantly. (C) 2015 Elsevier B.V. All rights reserved.
关键词	Bufadienolides Normal-phase Liquid Chromatography Two-dimensional Liquid Chromatography Ms/ms Toad Skin
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.jchromb.2015.11.015
关键词[WOS]	BUFO-GARGARIZANS CANTOR ; EFFICIENT PURIFICATION ; CARDIAC-GLYCOSIDES ; CHAN-SU ; ACTIVE BUFADIENOLIDES ; CELL-GROWTH ; HPLC-DAD ; STEROIDS ; CANCER ; IDENTIFICATION
收录类别	SCI
语种	英语
项目资助者	National Science Foundation of China(21305138) ; External Cooperation Program of BIC, Chinese Academy of Science(121421KYSB20130013) ; Key Projects in the National Science & Technology Pillar Program in the twelfth Five-year Plan(2012BAI29B08)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000379636800008
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6472
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 3.Wuhan Univ, Zhongnan Hosp, Dept Internal Med, Div Cardiol, Wuhan 430071, Hubei Province, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yun,Jin, Hongli,Li, Xiaolong,et al. Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1026:67-74.
APA	Zhang, Yun.,Jin, Hongli.,Li, Xiaolong.,Zhao, Jianqiang.,Guo, Xiujie.,...&Liang, Xinmiao.(2016).Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1026,67-74.
MLA	Zhang, Yun,et al."Separation and characterization of bufadienolides in toad skin using two-dimensional normal-phase liquid chromatography x reversed-phase liquid chromatography coupled with mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1026(2016):67-74.