Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC | |
Luan, Guangxiang1,3; Wang, Honglun1,2; Lv, Huanhuan1,3; Hu, Na1; Suo, Yourui1; Wang, Xiaoyan1 | |
2016-07-01 | |
发表期刊 | CHROMATOGRAPHIA |
卷号 | 79期号:13-14页码:823-831 |
文章类型 | Article |
摘要 | A method of using high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase liquid chromatography (semi-preparative RPLC) to preparatively separate flavone glucosides and lignan from the crude extracts of Caragana korshinskii has been established for the first time in this study. Five flavone glucosides, including 9 mg of kaempferol 3-O-{beta-d-glucopyranosyl(1 -> 2)-[alpha-l-rhamonopyranosyl(1 -> 6)]-beta-d-galactopyranoside}, 21 mg of kaempferol 3-O-alpha-l-rhamnopyranosyl(1 -> 6)-beta-d-galactopyranoside-7-O-alpha-l-rhamnopyranoside, 34 mg of kaempferol 3-O-beta-d-galactopyranoside-7-O-alpha-l-rhamnopyranoside, 27 mg of kaempferol 3-O-beta-d-glucopyranosyl-7-O-alpha-l-rhamnopyranoside, and 14 mg of calycosin 7-O-beta-d-glucopyranoside, and one lignan, 16 mg of alangilignoside B, were successfully isolated from 1.8 g of the crude sample through the combination of HSCCC with a two-phase solvent system composed of ethyl acetate-n-butanol-0.5 % formic acid (4:1:5, v/v/v) and semi-preparative RPLC with a mobile phase of methanol-water (20:80, v/v). The purities of the six compounds are all over 95 % as determined by HPLC and the structures are confirmed by the analysis of UV, H-1-NMR, and C-13-NMR and compared with published data. |
关键词 | Hsccc Semi-preparative Rplc Caragana Korshinskii Kom Flavone Glucosides Lignan Separation |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1007/s10337-016-3090-4 |
关键词[WOS] | COUNTER-CURRENT CHROMATOGRAPHY ; GLYCOSIDES ; ISOFLAVONES ; CHINENSIS ; SHIFT ; SEEDS ; HPLC |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Science Foundation of China(31470426 ; Qinghai Provincial Science Foundation(2015-SF-121 ; "The Dawn of West China" Talent Foundation of CAS ; Youth Innovation Promotion Association, CAS ; 31300292) ; 2015-ZJ-728 ; 2014-Z-768) |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS记录号 | WOS:000379157500003 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6475 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Huzhou Plateau Biol Resource Ctr Innovat, Huzhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Luan, Guangxiang,Wang, Honglun,Lv, Huanhuan,et al. Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC[J]. CHROMATOGRAPHIA,2016,79(13-14):823-831. |
APA | Luan, Guangxiang,Wang, Honglun,Lv, Huanhuan,Hu, Na,Suo, Yourui,&Wang, Xiaoyan.(2016).Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC.CHROMATOGRAPHIA,79(13-14),823-831. |
MLA | Luan, Guangxiang,et al."Separation and Purification of Five Flavone Glucosides and One Lignan from Caragana korshinskii Kom. by the Combination of HSCCC and Semi-preparative RPLC".CHROMATOGRAPHIA 79.13-14(2016):823-831. |
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