Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase

doi:10.1002/jssc.201500848

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	Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase
	Zhang, Qiulong 1,2; Luan, Guangxiang 1,2; Ma, Tao 1,2; Hu, Na 1,2; Suo, Yourui 1; Wang, Xiaoyan 1; Ma, Xiaofeng 2; Ding, Chenxu 1,3,4
	2015-12-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	38 期号:23 页码:4063-4070
文章类型	Article
摘要	A method that involved the combination of pH-zone-refining counter-current chromatography and semipreparative reversed-phase liquid chromatography has been established for the preparative separation of alkaloids from Hypecoum leptocarpum. From 1.2 g of crude sample, 31 mg N-feruloyltyramine, 27 mg oxohydrastinine, 47 mg hydroprotopine, 25 mg leptopidine, and 18 mg hypecocarpine have been obtained. The structure of the new compound, hypecocarpine, is confirmed based on the analysis of spectroscopic data, including NMR, UV, and IR spectroscopy and positive electrospray ionization mass spectrometry. The known chemical structures were characterized on the basis of H-1 and C-13 NMR spectroscopy. The purities of the five alkaloids are all over 92.7% as determined by high-performance liquid chromatography. The alkaloids' cytotoxicity in breast cancer cells is assessed by using a Cell Counting Kit assay and their inhibitory effect on fatty acid synthase expression is assessed by a Western blot assay. These results suggest that leptopidine could suppress growth and induce cytotoxicity in breast cancer cells and that the cytotoxicity of leptopidine may be related to its inhibitory effect on fatty acid synthase expression.
关键词	Alkaloids Fatty Acid Synthase Hypecoum Leptocarpum Ph-zone-refining Counter-current Chromatography
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1002/jssc.201500848
关键词[WOS]	COUNTER-CURRENT CHROMATOGRAPHY ; TETRAHYDROISOQUINOLINE DERIVATIVES ; BREAST-CANCER ; DISCOVERY ; POTENT ; AGENTS ; CELLS ; THOMS
收录类别	SCI
语种	英语
项目资助者	Key Project of Chinese Ministry of Education(212188) ; Bureau of Science and Technology(2014-HZ-810) ; Qinghai Provincial Natural Science Foundation(2015-ZJ-728) ; State Key Laboratory Breeding Base (Key Laboratory for Plateau Crop Germplasm Innovation and Utilization of Qinghai Province)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000367670000011
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6497
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qinghai Nationalities Univ, Xining, Peoples R China 4.Key Lab Plateau Crop Germplasm Innovat & Utilizat, Xining, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Qiulong,Luan, Guangxiang,Ma, Tao,et al. Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(23):4063-4070.
APA	Zhang, Qiulong.,Luan, Guangxiang.,Ma, Tao.,Hu, Na.,Suo, Yourui.,...&Ding, Chenxu.(2015).Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase.JOURNAL OF SEPARATION SCIENCE,38(23),4063-4070.
MLA	Zhang, Qiulong,et al."Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase".JOURNAL OF SEPARATION SCIENCE 38.23(2015):4063-4070.

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