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Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase
Zhang, Qiulong1,2; Luan, Guangxiang1,2; Ma, Tao1,2; Hu, Na1,2; Suo, Yourui1; Wang, Xiaoyan1; Ma, Xiaofeng2; Ding, Chenxu1,3,4
2015-12-01
Source PublicationJOURNAL OF SEPARATION SCIENCE
Volume38Issue:23Pages:4063-4070
SubtypeArticle
AbstractA method that involved the combination of pH-zone-refining counter-current chromatography and semipreparative reversed-phase liquid chromatography has been established for the preparative separation of alkaloids from Hypecoum leptocarpum. From 1.2 g of crude sample, 31 mg N-feruloyltyramine, 27 mg oxohydrastinine, 47 mg hydroprotopine, 25 mg leptopidine, and 18 mg hypecocarpine have been obtained. The structure of the new compound, hypecocarpine, is confirmed based on the analysis of spectroscopic data, including NMR, UV, and IR spectroscopy and positive electrospray ionization mass spectrometry. The known chemical structures were characterized on the basis of H-1 and C-13 NMR spectroscopy. The purities of the five alkaloids are all over 92.7% as determined by high-performance liquid chromatography. The alkaloids' cytotoxicity in breast cancer cells is assessed by using a Cell Counting Kit assay and their inhibitory effect on fatty acid synthase expression is assessed by a Western blot assay. These results suggest that leptopidine could suppress growth and induce cytotoxicity in breast cancer cells and that the cytotoxicity of leptopidine may be related to its inhibitory effect on fatty acid synthase expression.
KeywordAlkaloids Fatty Acid Synthase Hypecoum Leptocarpum Ph-zone-refining Counter-current Chromatography
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1002/jssc.201500848
WOS KeywordCOUNTER-CURRENT CHROMATOGRAPHY ; TETRAHYDROISOQUINOLINE DERIVATIVES ; BREAST-CANCER ; DISCOVERY ; POTENT ; AGENTS ; CELLS ; THOMS
Indexed BySCI
Language英语
Funding OrganizationKey Project of Chinese Ministry of Education(212188) ; Bureau of Science and Technology(2014-HZ-810) ; Qinghai Provincial Natural Science Foundation(2015-ZJ-728) ; State Key Laboratory Breeding Base (Key Laboratory for Plateau Crop Germplasm Innovation and Utilization of Qinghai Province)
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000367670000011
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/6497
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Qinghai Nationalities Univ, Xining, Peoples R China
4.Key Lab Plateau Crop Germplasm Innovat & Utilizat, Xining, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qiulong,Luan, Guangxiang,Ma, Tao,et al. Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase[J]. JOURNAL OF SEPARATION SCIENCE,2015,38(23):4063-4070.
APA Zhang, Qiulong.,Luan, Guangxiang.,Ma, Tao.,Hu, Na.,Suo, Yourui.,...&Ding, Chenxu.(2015).Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase.JOURNAL OF SEPARATION SCIENCE,38(23),4063-4070.
MLA Zhang, Qiulong,et al."Application of chromatography technology in the separation of active alkaloids from Hypecoum leptocarpum and their inhibitory effect on fatty acid synthase".JOURNAL OF SEPARATION SCIENCE 38.23(2015):4063-4070.
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