Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Ultrasound-assisted dispersive liquid-liquid microextraction method combined with HPLC-fluorescence detection for the determination of glycyrrhetinic acid in liquorice and liquorice-derived food products | |
You, Jinmao1,2; Wu, Hongliang1,2; Li, Guoliang1; Xia, Lian1; Zhao, Mei1; Lu, Shuaimin1; Kong, Weiheng1 | |
2016-02-01 | |
发表期刊 | JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
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卷号 | 13期号:2页码:359-367 |
文章类型 | Article |
摘要 | A sensitive and inexpensive method involving ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME) and pre-column derivatization followed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the analysis of glycyrrhetinic acid. In this work, glycyrrhetinic acid could be obtained by hydrolyzing glycyrrhizic acid to remove glucuronic acid and subsequently extracted by UA-DLLME using chloroform and acetone as the extraction and disperser solvents, respectively. The sample extraction was firstly concentrated to dry under nitrogen and then rapidly derivatized with 2-(12-oxobenzo[b]acridin-5(12H)-yl)-ethyl-4-toluenesulfonate (BAETS) after the UA-DLLME. The prime parameters influencing the UA-DLLME and derivatization procedure were optimized using response surface methodology. Under the optimum conditions, the proposed method has a better linearity in a wider range of 6-300 ng mL(-1) and a high square of correlation coefficient (R (2)) at 0.9994. Limit of detection and limit of quantification were found to be 1.7 ng mL(-1) and 5.8 ng mL(-1), respectively. The proposed method was applied to the analysis of glycyrrhetinic acid in liquorice, liquorice apricot and sugar plum samples. For the analysis of the spiked samples, the spiked recoveries were in the range of 90.4-103.0 % with RSD less than 5.18 %. All results demonstrated that the UA-DLLME-HPLC-FLD (ultrasound-assisted dispersive liquid-liquid microextraction-high-performance liquid chromatography with fluorescence detection) was a sensitive, accurate, efficient analytical method for the determination of glycyrrhetinic acid. |
关键词 | Liquorice Glycyrrhetinic Acid Ultrasound-assisted Dispersive Liquid-liquid Microextraction Pre-column Derivatization |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1007/s13738-015-0744-3 |
关键词[WOS] | GLYCYRRHIZA-URALENSIS FISCH ; 18-BETA-GLYCYRRHETINIC ACID ; GLABRA ; ACTIVATION ; EXTRACTION ; ROOTS ; WATER |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(31301595 ; Scientific Research Fund of Qufu Normal University(xkj201302) ; PhD research start-up funds of Qufu Normal University ; 21475074 ; 21275089) |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000367457900018 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6508 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Peoples R China |
推荐引用方式 GB/T 7714 | You, Jinmao,Wu, Hongliang,Li, Guoliang,et al. Ultrasound-assisted dispersive liquid-liquid microextraction method combined with HPLC-fluorescence detection for the determination of glycyrrhetinic acid in liquorice and liquorice-derived food products[J]. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY,2016,13(2):359-367. |
APA | You, Jinmao.,Wu, Hongliang.,Li, Guoliang.,Xia, Lian.,Zhao, Mei.,...&Kong, Weiheng.(2016).Ultrasound-assisted dispersive liquid-liquid microextraction method combined with HPLC-fluorescence detection for the determination of glycyrrhetinic acid in liquorice and liquorice-derived food products.JOURNAL OF THE IRANIAN CHEMICAL SOCIETY,13(2),359-367. |
MLA | You, Jinmao,et al."Ultrasound-assisted dispersive liquid-liquid microextraction method combined with HPLC-fluorescence detection for the determination of glycyrrhetinic acid in liquorice and liquorice-derived food products".JOURNAL OF THE IRANIAN CHEMICAL SOCIETY 13.2(2016):359-367. |
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