Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis

NWIPB OpenIR

	Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis
	Yang, Renming 1; Wang, Honglun 1; Li, Gang 2; Suo, Yourui 1; You, Jinmao 3; Wang, HL (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China.
	2014-08-01
发表期刊	ASIAN JOURNAL OF CHEMISTRY
卷号	26 期号:15 页码:4830-4834
文章类型	Article
摘要	In this study, a central composite design based on response surface methodology was used to optimize ultrasonic-assistant extraction of flavonoids from Trigonella foenum-graecum L. The optimal conditions to obtain the highest yield of total flavonoids were as follows: ultrasonic time of 80 min, temperature of 48 degrees C and liquid to solid ratio of 20 g/mL, where 1.03 % yield was predicted. After extraction process optimization, a method for the analysis of flavonoids in Trigonella foenum-graecum L. by HPLC-DAD-MS was developed. The LOD and LOQ of these 4 compounds range from 50.94 to 70.31 ng/mL and 179.63 to 208.85 ng/mL, respectively. The established HPLC-DAD-MS method was proven to be useful for the quantitative and qualitative analysis of flavonoid constituents in fenugreek seed. This is the first report on preparation, identification and determination of flavonoids in fenugreek seed by different method and which would provide a basis for medicinal and edible use of fenugreek.
关键词	Fenugreek Flavonoids Response Surface Methodology Ultrasound-assisted Extraction Quantitative Analysis
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	TRIGONELLA-FOENUM-GRAECUM ; SEEDS ; GLYCOSIDES ; PHENOLICS
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000343766300062
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6597
专题	中国科学院西北高原生物研究所
通讯作者	Wang, HL (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China.
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Yantai Univ, Life Sch, Yantai 264005, Peoples R China 3.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Yang, Renming,Wang, Honglun,Li, Gang,et al. Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis[J]. ASIAN JOURNAL OF CHEMISTRY,2014,26(15):4830-4834.
APA	Yang, Renming,Wang, Honglun,Li, Gang,Suo, Yourui,You, Jinmao,&Wang, HL .(2014).Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis.ASIAN JOURNAL OF CHEMISTRY,26(15),4830-4834.
MLA	Yang, Renming,et al."Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis".ASIAN JOURNAL OF CHEMISTRY 26.15(2014):4830-4834.