Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis | |
Yang, Renming1; Wang, Honglun1; Li, Gang2; Suo, Yourui1; You, Jinmao3; Wang, HL (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China. | |
2014-08-01 | |
发表期刊 | ASIAN JOURNAL OF CHEMISTRY
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卷号 | 26期号:15页码:4830-4834 |
文章类型 | Article |
摘要 | In this study, a central composite design based on response surface methodology was used to optimize ultrasonic-assistant extraction of flavonoids from Trigonella foenum-graecum L. The optimal conditions to obtain the highest yield of total flavonoids were as follows: ultrasonic time of 80 min, temperature of 48 degrees C and liquid to solid ratio of 20 g/mL, where 1.03 % yield was predicted. After extraction process optimization, a method for the analysis of flavonoids in Trigonella foenum-graecum L. by HPLC-DAD-MS was developed. The LOD and LOQ of these 4 compounds range from 50.94 to 70.31 ng/mL and 179.63 to 208.85 ng/mL, respectively. The established HPLC-DAD-MS method was proven to be useful for the quantitative and qualitative analysis of flavonoid constituents in fenugreek seed. This is the first report on preparation, identification and determination of flavonoids in fenugreek seed by different method and which would provide a basis for medicinal and edible use of fenugreek. |
关键词 | Fenugreek Flavonoids Response Surface Methodology Ultrasound-assisted Extraction Quantitative Analysis |
WOS标题词 | Science & Technology ; Physical Sciences |
关键词[WOS] | TRIGONELLA-FOENUM-GRAECUM ; SEEDS ; GLYCOSIDES ; PHENOLICS |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000343766300062 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6597 |
专题 | 中国科学院西北高原生物研究所 |
通讯作者 | Wang, HL (reprint author), Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China. |
作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China 2.Yantai Univ, Life Sch, Yantai 264005, Peoples R China 3.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, Renming,Wang, Honglun,Li, Gang,et al. Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis[J]. ASIAN JOURNAL OF CHEMISTRY,2014,26(15):4830-4834. |
APA | Yang, Renming,Wang, Honglun,Li, Gang,Suo, Yourui,You, Jinmao,&Wang, HL .(2014).Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis.ASIAN JOURNAL OF CHEMISTRY,26(15),4830-4834. |
MLA | Yang, Renming,et al."Optimization of Ultrasonic-Assisted Extraction of Flavonoids from Fenugreek Using Response Surface Methodology and High-Performance Liquid Chromatography-Diode Array Detector Coupled with Mass Spectrometry Analysis".ASIAN JOURNAL OF CHEMISTRY 26.15(2014):4830-4834. |
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