Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide

doi:10.1016/j.talanta.2016.08.040

NWIPB OpenIR

	Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide
	Lv, Zhengxian 1,2; Sun, Zhiwei 1,2; Song, Cuihua 1,2; Lu, Shuaimin 1,2; Chen, Guang 1,2; You, Jinmao 1,2,3
	2016-12-01
发表期刊	TALANTA
卷号	161 页码:228-237
文章类型	Article
摘要	A sensitive and background-free pre-column derivatization method for the determination of thiol compounds using metal-organic framework material (MOF-5) as dispersive solid-phase extraction (DSPE) adsorbent followed by high-performance liquid chromatography fluorescence detection (HPLC-FLD) has been developed. In this paper, a novel labeling reagent, carbazole-9-ethyl-2-maleimide(CAEM), was synthesized and reacted with thiols at 40 degrees C for 10 min in the presence of PBS buffer (0.02 mol/L, pH 7.5). Interestingly, CAEM itself had no fluorescence, while its derivatives exhibited intense fluorescence with an excitation maximum at lambda(ex) 274 nm and an emission maximum at lambda(em) 363 nm, which greatly reduced the background interference and improved the sensitivity of the method. Furthermore, the MOF-5 was prepared and used as DSPE adsorbent for the selective adsorption of thiols from wastewater sample. Under the optimized experimental conditions, an excellent linearity for all analytes over their concentration ranges of 0.01-1.0 mu mol/L (R-2 > 0.9986)were obtained with the limit of detection (LOD) ranging from 8 to 17.1 pmol/L for nine tested thiols. The feasibility of this method for the determination of thiols in wastewater samples had beep evaluated and satisfactory average recoveries (n=3) were achieved with the range of 86.6-98.5%. (C) 2016 Elsevier B.V. All rights reserved.
关键词	Thiols Carbazole-9-ethyl-2-maleimide (Caem) High-performance Liquid Chromatography (Hplc) Mof-5 Wastewater
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1016/j.talanta.2016.08.040
关键词[WOS]	SOLID-PHASE EXTRACTION ; METAL-ORGANIC FRAMEWORK ; SPECTROFLUOROMETRIC DETERMINATION ; CAPILLARY-ELECTROPHORESIS ; ELECTROCHEMICAL DETECTION ; PRECOLUMN DERIVATIZATION ; GAS CHROMATOGRAPHY ; PLASMA ; HPLC ; MICROEXTRACTION
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21475075 ; National Natural Science Foundation of Shandong Province(ZR2015CM040) ; 21475074 ; 21305076 ; 21275089)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000386989500029
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6668
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med S, Qufu 273165, Peoples R China 3.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Lv, Zhengxian,Sun, Zhiwei,Song, Cuihua,et al. Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide[J]. TALANTA,2016,161:228-237.
APA	Lv, Zhengxian,Sun, Zhiwei,Song, Cuihua,Lu, Shuaimin,Chen, Guang,&You, Jinmao.(2016).Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide.TALANTA,161,228-237.
MLA	Lv, Zhengxian,et al."Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide".TALANTA 161(2016):228-237.