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Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide
Lv, Zhengxian1,2; Sun, Zhiwei1,2; Song, Cuihua1,2; Lu, Shuaimin1,2; Chen, Guang1,2; You, Jinmao1,2,3
2016-12-01
Source PublicationTALANTA
Volume161Pages:228-237
SubtypeArticle
AbstractA sensitive and background-free pre-column derivatization method for the determination of thiol compounds using metal-organic framework material (MOF-5) as dispersive solid-phase extraction (DSPE) adsorbent followed by high-performance liquid chromatography fluorescence detection (HPLC-FLD) has been developed. In this paper, a novel labeling reagent, carbazole-9-ethyl-2-maleimide(CAEM), was synthesized and reacted with thiols at 40 degrees C for 10 min in the presence of PBS buffer (0.02 mol/L, pH 7.5). Interestingly, CAEM itself had no fluorescence, while its derivatives exhibited intense fluorescence with an excitation maximum at lambda(ex) 274 nm and an emission maximum at lambda(em) 363 nm, which greatly reduced the background interference and improved the sensitivity of the method. Furthermore, the MOF-5 was prepared and used as DSPE adsorbent for the selective adsorption of thiols from wastewater sample. Under the optimized experimental conditions, an excellent linearity for all analytes over their concentration ranges of 0.01-1.0 mu mol/L (R-2 > 0.9986)were obtained with the limit of detection (LOD) ranging from 8 to 17.1 pmol/L for nine tested thiols. The feasibility of this method for the determination of thiols in wastewater samples had beep evaluated and satisfactory average recoveries (n=3) were achieved with the range of 86.6-98.5%. (C) 2016 Elsevier B.V. All rights reserved.
KeywordThiols Carbazole-9-ethyl-2-maleimide (Caem) High-performance Liquid Chromatography (Hplc) Mof-5 Wastewater
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1016/j.talanta.2016.08.040
WOS KeywordSOLID-PHASE EXTRACTION ; METAL-ORGANIC FRAMEWORK ; SPECTROFLUOROMETRIC DETERMINATION ; CAPILLARY-ELECTROPHORESIS ; ELECTROCHEMICAL DETECTION ; PRECOLUMN DERIVATIZATION ; GAS CHROMATOGRAPHY ; PLASMA ; HPLC ; MICROEXTRACTION
Indexed BySCI
Language英语
Funding OrganizationNational Natural Science Foundation of China(21475075 ; National Natural Science Foundation of Shandong Province(ZR2015CM040) ; 21475074 ; 21305076 ; 21275089)
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000386989500029
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/6668
Collection中国科学院西北高原生物研究所
Affiliation1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China
2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med S, Qufu 273165, Peoples R China
3.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China
Recommended Citation
GB/T 7714
Lv, Zhengxian,Sun, Zhiwei,Song, Cuihua,et al. Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide[J]. TALANTA,2016,161:228-237.
APA Lv, Zhengxian,Sun, Zhiwei,Song, Cuihua,Lu, Shuaimin,Chen, Guang,&You, Jinmao.(2016).Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide.TALANTA,161,228-237.
MLA Lv, Zhengxian,et al."Sensitive and background-free determination of thiols from wastewater samples by MOF-5 extraction coupled with high-performance liquid chromatography with fluorescence detection using a novel fluorescence probe of carbazole-9-ethyl-2-maleimide".TALANTA 161(2016):228-237.
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