Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Mass spectrometric identification of aliphatic amines using 2-(11H-Benzo[a] carbazol-11-yl) ethyl chloroformate as fluorescence labeling reagent | |
Zhao Wen-Chen; Liu Ling-Jun; Zhu Fang; Zhao Xian-En; Liu Su-Juan; Suo You-Rui; You Jin-Mao | |
2007-09-01 | |
发表期刊 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY |
卷号 | 35期号:9页码:1251-1256 |
文章类型 | Article |
摘要 | A simple and highly sensitive method based on the pre-column derivatization for the determination of aliphatic amines in food using a nitrogen -containing 2-( 1 1H-benzo [ a] carbazol-11-yl) ethyl chloroformate BCEC-Cl) as fluorescence labeling reagent followed by high-performance liquid chromatography with fluorescence detection has been developed. The stable derivatives can be obtained by the labeling reaction of BCEC-Cl with amines in the presence of borate buffer ( pH 9. 0) within 3 min. The maximum excitation and emission wavelengths were 279 nm of lambda(ex) and 380 nm of lambda(em) respectively. Separation of 12 amine-derivatives was achieved by a gradient elution on a reversed-phase Eclipse XDB C-8 ( 150 min x 4. 6 mm, 5 mu m) column with a good baseline resolution. The identification of amine -derivatives was carried out by the online post-column MS using atmospheric pressure chemical ionization (APCI) as ion source in positive-Ion detection mode. The established method exhibits excellent reproducibility and recovery. Excellent linear responses were observed with the correlation coefficients > 0. 9996. The detection I units ( at signal-to-noise of 3 : 1) were at levels 1. 8 - 14 fmol. |
关键词 | High Performance Liquid Chromatography-mass Spectrometry Fluorescence Detection Pre-column Derivatization Aliphatic Amines |
WOS标题词 | Science & Technology ; Physical Sciences |
关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; PRECOLUMN DERIVATIZATION ; ACIDS |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000251296200003 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6862 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Sky Lab Life Organ Anal, Qufu 273165, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao Wen-Chen,Liu Ling-Jun,Zhu Fang,et al. Mass spectrometric identification of aliphatic amines using 2-(11H-Benzo[a] carbazol-11-yl) ethyl chloroformate as fluorescence labeling reagent[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(9):1251-1256. |
APA | Zhao Wen-Chen.,Liu Ling-Jun.,Zhu Fang.,Zhao Xian-En.,Liu Su-Juan.,...&You Jin-Mao.(2007).Mass spectrometric identification of aliphatic amines using 2-(11H-Benzo[a] carbazol-11-yl) ethyl chloroformate as fluorescence labeling reagent.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,35(9),1251-1256. |
MLA | Zhao Wen-Chen,et al."Mass spectrometric identification of aliphatic amines using 2-(11H-Benzo[a] carbazol-11-yl) ethyl chloroformate as fluorescence labeling reagent".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 35.9(2007):1251-1256. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论