Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization | |
Ding Yang-Jun; Zhao Xian-En; Zhu Fang; Suo You-Rui; Li Yu-Lin; Chen Cui-Chen; You Jin-Mao | |
2007-03-01 | |
发表期刊 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY |
卷号 | 35期号:3页码:375-381 |
文章类型 | Article |
摘要 | A method for the identification of long-chain unsaturated fatty acids in deep-sea fish oil using 1 2-benzo-3, 4-dihydrocarbazole-9-ethyl-benzenesulfonte (BDEBS) as pre-column derivatization reagent by reversed-phase HPLC/MS has been developed. To avoid the migration of double bond in the fatty acid chain, fish oil samples were saponificated and derivatized under nitrogen circumstance. The derivatized solution was extracted with hexane and dried under a stream of nitrogen; the residue was re-dissolved with acetorntrile prior to LC analysis. MS analysis of the fatty acid derivatives exhibited regular information regarding the position of the double bond in the fatty acid chain. With molecular foil mass at m/z [MH](+) and specific fragment ions obtained with MS analysis, the position of the double bond in fatty acid chain was located (or calculated) by means of several established formulas. According to the analysis of HPLC/MS/APCI, 23 fatty acids were identified. The main fatty acids in deep-sea fish oil are C-14-C-22 and 67.08% (area percent) of them are polytinsaturated fatly acids. The main components of the unsaturated fatty acids are C(16:1)9-hexadecenoic acid, 11.7% C16:4 4, 7, 10, 13-hexadecatetraenoic acid, 2.91%; C-18:1 12-octadecenoic acid, 11.1%; C-20:0 6, 9, 12, 15-octadecatetractioic acid, 3.62%; C-20:1 13-eicosenoic acid, 1.21%; C(20:5)5, 8, 11, 14, 17-eicosapentaenoic acid, 16.71%; C(22:6)2, 5, 8, 11, 14, 17-docosahexenoic acid, 10. 53%. The established method provides a new technical tool for the location of doulde bond positions in the, poly unsaturated fatty acids. |
关键词 | High Performance Liquid Chromatography/mass Spectrometry Pre-column Derivatization Fish Oil Fatty Acids Position Of Double Bond |
WOS标题词 | Science & Technology ; Physical Sciences |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000245735200013 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/6867 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China |
推荐引用方式 GB/T 7714 | Ding Yang-Jun,Zhao Xian-En,Zhu Fang,et al. Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2007,35(3):375-381. |
APA | Ding Yang-Jun.,Zhao Xian-En.,Zhu Fang.,Suo You-Rui.,Li Yu-Lin.,...&You Jin-Mao.(2007).Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,35(3),375-381. |
MLA | Ding Yang-Jun,et al."Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 35.3(2007):375-381. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论