Determination of Fatty Acids (C-1-C-10) from Bryophytes and Pteridophytes

NWIPB OpenIR

	Determination of Fatty Acids (C-1-C-10) from Bryophytes and Pteridophytes
	Bai, Xin-Wei 1; Song, Cui-Hua 1; You, Jin-Mao 1,2; Sun, Zhi-Wei 1,2; Fu, Yan-Yan 1; Li, Guo-Liang 2
	2010-06-01
发表期刊	CHROMATOGRAPHIA
卷号	71 期号:11-12 页码:1125-1129
文章类型	Article
摘要	A simple and mild method for the determination of fatty acids (C-1 - C-10) based on a condensation reaction using 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) as labeling reagent with capillary zone electrophoresis has been developed. The detection was performed with a diode array detector at 254 nm. A 58.5 cm x 50 mu m i.d. (50 cm effective length) untreated fused-silica capillary was used. To optimize the separation conditions, the background electrolyte concentration, column temperature, voltage and other factors were evaluated. The optimal separation conditions were as follows: 30 mmol L-1 borate buffer (pH 9.5), 15 mmol L-1 beta-CD, temperature at 20 A degrees C, pressure 50 mbar and injection time 8 s. Under the established conditions, 10 fatty acid derivatives could be well-separated within 17 min. The linearity was in the range of 0.07-5.0 mu mol L-1. Detection limits (at a signal-to-noise ratio of 3) were in the range of 0.027-0.042 mu mol L-1. The fatty acids from the extracted Funaria Hedw. and Selaginella samples were determined with satisfactory results.
关键词	Capillary Zone Electrophoresis Pre-column Derivation Fatty Acids Funaria Hedw. And Selaginella
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]	CAPILLARY-ELECTROPHORESIS ; FLUORESCENCE DETECTION ; LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; LABELING REAGENT ; IDENTIFICATION ; LIVERWORTS ; JAPANESE
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000277936600023
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6888
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Bai, Xin-Wei,Song, Cui-Hua,You, Jin-Mao,et al. Determination of Fatty Acids (C-1-C-10) from Bryophytes and Pteridophytes[J]. CHROMATOGRAPHIA,2010,71(11-12):1125-1129.
APA	Bai, Xin-Wei,Song, Cui-Hua,You, Jin-Mao,Sun, Zhi-Wei,Fu, Yan-Yan,&Li, Guo-Liang.(2010).Determination of Fatty Acids (C-1-C-10) from Bryophytes and Pteridophytes.CHROMATOGRAPHIA,71(11-12),1125-1129.
MLA	Bai, Xin-Wei,et al."Determination of Fatty Acids (C-1-C-10) from Bryophytes and Pteridophytes".CHROMATOGRAPHIA 71.11-12(2010):1125-1129.