Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC

doi:10.1007/s10337-017-3285-3

NWIPB OpenIR

	Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC
	Xia, Lian 1; Liu, Lijie 1; Qu, Fengli 1; Kong, Rongmei 1; Li, Guoliang 1; You, Jinmao 1,2
	2017-06-01
发表期刊	CHROMATOGRAPHIA
卷号	80 期号:6 页码:861-872
文章类型	Article
摘要	The presence or absence of acetyl groups in oligosaccharides greatly affects the biological activities of glycoproteins. Consequently, in studies on glycoproteins, sensitive and accurate methods are required for the analysis of acetylated amino sugars (AASs) are required. We report here our newly developed method for the determination of four AASs [N-acetyl-d-glucosamine (GlcNAc), N-acetyl-d-galactosamine (GalNAc), N-acetyl neuraminic acid (Neu5Ac), N-glycolyl neuraminic acid (Neu5Gc)]. This method is based on the optimization of the release of AASs from Potentilla anserina L. glycoprotein and involves modification of experimental design and the use of high-performance liquid chromatography coupled with UV detection and mass spectrometry identification. Using our optimized conditions of derivatization and separation, we successfully detected these four AAS derivatives with good precision and accuracy. This is the first report of determination of the four AASs simultaneously on the basis of its release study. The newly developed method exhibits a high potential for the analysis of AASs from various bio-samples and provides a sensitive method for further study of the relationship between AAS composition and their bioactivities in glycoprotein.
关键词	Hplc Response Surface Methodology Acetylated Amino Sugar Glycoprotein Release Condition
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1007/s10337-017-3285-3
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS ; MONOSACCHARIDE COMPOSITION ; CARBOHYDRATE ANALYSIS ; MASS-SPECTROMETRY ; SIALIC-ACID ; DERIVATIZATION ; POLYSACCHARIDES ; GANODERMA ; EXTRACT
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(21505084 ; Natural Science Foundation of Shandong Province(ZR2014BM029) ; 21475075 ; 21375076 ; 31301595)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000402824400004
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/7015
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 2.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Xia, Lian,Liu, Lijie,Qu, Fengli,et al. Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC[J]. CHROMATOGRAPHIA,2017,80(6):861-872.
APA	Xia, Lian,Liu, Lijie,Qu, Fengli,Kong, Rongmei,Li, Guoliang,&You, Jinmao.(2017).Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC.CHROMATOGRAPHIA,80(6),861-872.
MLA	Xia, Lian,et al."Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC".CHROMATOGRAPHIA 80.6(2017):861-872.