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题名: Optimization of Release Conditions for Acetylated Amino Sugars from Glycoprotein with the Aid of Experimental Design and Their Sensitive Determination with HPLC
作者: Xia, Lian1; Liu, Lijie1; Qu, Fengli1; Kong, Rongmei1; Li, Guoliang1; You, Jinmao1, 2
刊名: CHROMATOGRAPHIA
出版日期: 2017-06-01
卷号: 80, 期号:6, 页码:861-872
关键词: HPLC ; Response surface methodology ; Acetylated amino sugar ; Glycoprotein ; Release condition
DOI: 10.1007/s10337-017-3285-3
文章类型: Article
英文摘要: The presence or absence of acetyl groups in oligosaccharides greatly affects the biological activities of glycoproteins. Consequently, in studies on glycoproteins, sensitive and accurate methods are required for the analysis of acetylated amino sugars (AASs) are required. We report here our newly developed method for the determination of four AASs [N-acetyl-d-glucosamine (GlcNAc), N-acetyl-d-galactosamine (GalNAc), N-acetyl neuraminic acid (Neu5Ac), N-glycolyl neuraminic acid (Neu5Gc)]. This method is based on the optimization of the release of AASs from Potentilla anserina L. glycoprotein and involves modification of experimental design and the use of high-performance liquid chromatography coupled with UV detection and mass spectrometry identification. Using our optimized conditions of derivatization and separation, we successfully detected these four AAS derivatives with good precision and accuracy. This is the first report of determination of the four AASs simultaneously on the basis of its release study. The newly developed method exhibits a high potential for the analysis of AASs from various bio-samples and provides a sensitive method for further study of the relationship between AAS composition and their bioactivities in glycoprotein.
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; CAPILLARY-ELECTROPHORESIS ; MONOSACCHARIDE COMPOSITION ; CARBOHYDRATE ANALYSIS ; MASS-SPECTROMETRY ; SIALIC-ACID ; DERIVATIZATION ; POLYSACCHARIDES ; GANODERMA ; EXTRACT
收录类别: SCI
项目资助者: National Natural Science Foundation of China(21505084 ; Natural Science Foundation of Shandong Province(ZR2014BM029) ; 21475075 ; 21375076 ; 31301595)
语种: 英语
WOS记录号: WOS:000402824400004
Citation statistics:
内容类型: 期刊论文
URI标识: http://210.75.249.4/handle/363003/7015
Appears in Collections:中国科学院西北高原生物研究所_期刊论文

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作者单位: 1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China
2.Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China
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