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Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique | |
Pei, Jinjin1; Jiang, Lei2 | |
2017-07-01 | |
发表期刊 | INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
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卷号 | 50期号:1页码:41-46 |
文章类型 | Article |
摘要 | Andrias davidianus, the Chinese giant salamander, has been used in traditional Chinese medicine for many decades. However, no antimicrobial peptides (AMPs) have been described from A. davidianus until now. Here we describe a novel AMP (andricin 01) isolated from the mucus of A. davidianus. The peptide was recovered using an innovative magnetic cell membrane separation technique and was characterised using mass spectrometry and circular dichroism (CD) spectroscopy. Andricin 01 is comprised of ten amino acid residues with a total molecular mass of 955.1 Da. CD spectrum analysis gave results similar to the archetypal random coil spectrum, consistent with the three-dimensional rendering calculated by current bioinformatics tools. Andricin 01 was found to be inhibitory both to Gram-negative and Gram-positive bacteria. Furthermore, the peptide at the minimal bacterial concentration did not show cell cytotoxicity against human hepatocytes or renal cells and did not show haemolytic activity against red blood cells, indicating that is potentially safe and effective for human use. Andricin 01 shows promise as a novel antibacterial that may provide an insight into the development of new drugs. (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved. |
关键词 | Andrias Davidianus Mucus Antimicrobial Peptide Magnetic Cell Membrane Separation Escherichia Coli |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1016/j.ijantimicag.2017.02.013 |
关键词[WOS] | CHINESE ; CHROMATOGRAPHY ; SKINS |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Research Foundation of Science and Technology Bureau of Shaanxi, China(2015SZS-15-05) ; Research Foundation of Education Bureau of Shaanxi, China(16JS021) ; China Scholarship Council (CSC) Foundation(201608615024) ; Special Science Foundation of Shaanxi University of Technology(SLGKYQD2014-2-18) |
WOS研究方向 | Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy |
WOS类目 | Infectious Diseases ; Microbiology ; Pharmacology & Pharmacy |
WOS记录号 | WOS:000405983100007 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/7057 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Shaanxi Univ Technol, Shaanxi Key Lab Biol & Bioresources, Chaoyang Rd, Hanzhong 723001, Shaanxi, Peoples R China 2.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Pei, Jinjin,Jiang, Lei. Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique[J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS,2017,50(1):41-46. |
APA | Pei, Jinjin,&Jiang, Lei.(2017).Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique.INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS,50(1),41-46. |
MLA | Pei, Jinjin,et al."Antimicrobial peptide from mucus of Andrias davidianus: screening and purification by magnetic cell membrane separation technique".INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS 50.1(2017):41-46. |
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