Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography

doi:10.1002/jssc.201601134

NWIPB OpenIR

	Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography
	Li, Hongmei 1,2; Chen, Tao 1; Sun, Jing 1; Wang, Weiqing 3; Li, Yulin 1
	2017-06-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
卷号	40 期号:11 页码:2515-2521
文章类型	Article
摘要	In our present study, two groups of xanthones isomers (1-hydroxy-3,5,8-trimethoxyxanthone and 1-hydroxy-3,7,8-trimethoxyxanthone; 1,8-dihydroxy-3,7-dimethoxyxanthone and 1,8-dihydroxy-3,5-dimethanolxanthone) and other two xanthones (3-methoxy-1,5,8-trihydroxyxanthone and 3,5-dimethoxy-1-hydroxyxanthone) were separated from Swertia franchetiana. First, a solvent system composed of petroleum ether/methanol/water (2:1:0.6, v/v) was developed for the liquid-liquid extraction of these xanthones from the crude extract. Then, an efficient method was established for the one-step separation of these six xanthones by high-speed countercurrent chromatography using n-hexane/ethyl acetate/methanol/ethanol/water (HEMEW; 6:4:4:2:4, v/v) as the solvent system. The results showed that liquid-liquid extraction could be well developed for efficient enrichment of target compounds. Additionally, high-speed countercurrent chromatography could be a powerful technology for separation xanthones isomers. It was found ethanol could be a good methanol substitute when the HEMEW system could not provide good separation factors.
关键词	Countercurrent Chromatography Isomers Swertia Franchetiana Xanthones
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1002/jssc.201601134
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; 3 ANTHRAQUINONE GLYCOSIDES ; RHEUM-TANGUTICUM ; ULTRAFILTRATION-HPLC ; MACROPOROUS RESIN ; RAPID ENRICHMENT ; PURIFICATION ; EXTRACTION ; IDENTIFICATION ; INHIBITORS
收录类别	SCI
语种	英语
项目资助者	"Personalized Medicines-Molecular Signature-based Drug Discovery and Development," Strategic Priority Research Program of the Chinese Academy of Sciences(XDA12050304) ; National Natural Science Foundation of China(81403051) ; Instrument Function Development Project of CAS(2017g107)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000403461500023
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/7085
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining, Peoples R China 2.Univ Chinese Acad Sci, Savaid Med Sch, Beijing, Peoples R China 3.Qinghai Prov Prod Qual Supervis & Inspect Ctr, Xining, Peoples R China
推荐引用方式 GB/T 7714	Li, Hongmei,Chen, Tao,Sun, Jing,et al. Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(11):2515-2521.
APA	Li, Hongmei,Chen, Tao,Sun, Jing,Wang, Weiqing,&Li, Yulin.(2017).Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography.JOURNAL OF SEPARATION SCIENCE,40(11),2515-2521.
MLA	Li, Hongmei,et al."Separation of six xanthones from Swertia franchetiana by high-speed countercurrent chromatography".JOURNAL OF SEPARATION SCIENCE 40.11(2017):2515-2521.