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Cytological and molecular identification of alien chromatin in giant spike wheat germplasm | |
Dou, QW; Chen, PD; Xie, JF | |
2003-09-01 | |
发表期刊 | ACTA BOTANICA SINICA
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卷号 | 45期号:9页码:1109-1115 |
文章类型 | Article |
摘要 | Alien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding, genomic in situ hybridization (GISH), sequence characterized amplified region (SCAR), and random amplified polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried both a pair of wheat-rye (Triticum aestivum-Secale cereal) 1BL/1RS translocation chromosomes and a pair of Agropyron intermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three others carried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome. Further identification revealed that the identical Ai chromosome in these germplasm lines substituted the chromosome 2D of common wheat (Triticum aestivum L.), designated as 2Ai. The genetic implication and further utilization of 2Ai in wheat improvement were also discussed.; Alien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding, genomic in situ hybridization (GISH), sequence characterized amplified region (SCAR), and random amplified polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried both a pair of wheat-rye (Triticum aestivum-Secale cereal) 1BL/1RS translocation chromosomes and a pair of Agropyron intermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three others carried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome. Further identification revealed that the identical Ai chromosome in these germplasm lines substituted the chromosome 2D of common wheat (T aestivum L.), designated as 2Ai. The genetic implication and further utilization of 2Ai in wheat improvement were also discussed. |
关键词 | Giant Spike Germplasm 1 Bl/1 Rs Agropyron Intermedium C-bandIng Genomic In Situ Hybridization (Gish) Sequence Characterized Amplified Region (Scar) Random Amplified Polymorphic Dna (Rapd) |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
学科领域 | 农作物 |
关键词[WOS] | INSITU HYBRIDIZATION ; TRITICUM-AESTIVUM ; TETRAPLOID WHEAT ; RYE CHROMATIN ; RESISTANCE ; MARKERS |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
WOS记录号 | WOS:000186548600016 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/727 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Nanjing Agr Univ, Key Lab Crop Cytogenet, Minist Agr, Nanjing 210095, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810008, Peoples R China |
推荐引用方式 GB/T 7714 | Dou, QW,Chen, PD,Xie, JF. Cytological and molecular identification of alien chromatin in giant spike wheat germplasm[J]. ACTA BOTANICA SINICA,2003,45(9):1109-1115. |
APA | Dou, QW,Chen, PD,&Xie, JF.(2003).Cytological and molecular identification of alien chromatin in giant spike wheat germplasm.ACTA BOTANICA SINICA,45(9),1109-1115. |
MLA | Dou, QW,et al."Cytological and molecular identification of alien chromatin in giant spike wheat germplasm".ACTA BOTANICA SINICA 45.9(2003):1109-1115. |
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