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Cytological and molecular identification of alien chromatin in giant spike wheat germplasm
Dou, QW; Chen, PD; Xie, JF
2003-09-01
Source PublicationACTA BOTANICA SINICA
Volume45Issue:9Pages:1109-1115
SubtypeArticle
AbstractAlien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding, genomic in situ hybridization (GISH), sequence characterized amplified region (SCAR), and random amplified polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried both a pair of wheat-rye (Triticum aestivum-Secale cereal) 1BL/1RS translocation chromosomes and a pair of Agropyron intermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three others carried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome. Further identification revealed that the identical Ai chromosome in these germplasm lines substituted the chromosome 2D of common wheat (Triticum aestivum L.), designated as 2Ai. The genetic implication and further utilization of 2Ai in wheat improvement were also discussed.; Alien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding, genomic in situ hybridization (GISH), sequence characterized amplified region (SCAR), and random amplified polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried both a pair of wheat-rye (Triticum aestivum-Secale cereal) 1BL/1RS translocation chromosomes and a pair of Agropyron intermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three others carried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome. Further identification revealed that the identical Ai chromosome in these germplasm lines substituted the chromosome 2D of common wheat (T aestivum L.), designated as 2Ai. The genetic implication and further utilization of 2Ai in wheat improvement were also discussed.
KeywordGiant Spike Germplasm 1 Bl/1 Rs Agropyron Intermedium C-bandIng Genomic In Situ Hybridization (Gish) Sequence Characterized Amplified Region (Scar) Random Amplified Polymorphic Dna (Rapd)
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Subject Area农作物
WOS KeywordINSITU HYBRIDIZATION ; TRITICUM-AESTIVUM ; TETRAPLOID WHEAT ; RYE CHROMATIN ; RESISTANCE ; MARKERS
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Plant Sciences
WOS SubjectBiochemistry & Molecular Biology ; Plant Sciences
WOS IDWOS:000186548600016
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/727
Collection中国科学院西北高原生物研究所
Affiliation1.Nanjing Agr Univ, Key Lab Crop Cytogenet, Minist Agr, Nanjing 210095, Peoples R China
2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810008, Peoples R China
Recommended Citation
GB/T 7714
Dou, QW,Chen, PD,Xie, JF. Cytological and molecular identification of alien chromatin in giant spike wheat germplasm[J]. ACTA BOTANICA SINICA,2003,45(9):1109-1115.
APA Dou, QW,Chen, PD,&Xie, JF.(2003).Cytological and molecular identification of alien chromatin in giant spike wheat germplasm.ACTA BOTANICA SINICA,45(9),1109-1115.
MLA Dou, QW,et al."Cytological and molecular identification of alien chromatin in giant spike wheat germplasm".ACTA BOTANICA SINICA 45.9(2003):1109-1115.
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