Cytological and molecular identification of alien chromatin in giant spike wheat germplasm

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	Cytological and molecular identification of alien chromatin in giant spike wheat germplasm
	Dou, QW ; Chen, PD ; Xie, JF
	2003-09-01
发表期刊	ACTA BOTANICA SINICA
卷号	45 期号:9 页码:1109-1115
文章类型	Article
摘要	Alien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding, genomic in situ hybridization (GISH), sequence characterized amplified region (SCAR), and random amplified polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried both a pair of wheat-rye (Triticum aestivum-Secale cereal) 1BL/1RS translocation chromosomes and a pair of Agropyron intermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three others carried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome. Further identification revealed that the identical Ai chromosome in these germplasm lines substituted the chromosome 2D of common wheat (Triticum aestivum L.), designated as 2Ai. The genetic implication and further utilization of 2Ai in wheat improvement were also discussed.; Alien chromosomes of twelve giant spike wheat germplasm lines were identified by C-banding, genomic in situ hybridization (GISH), sequence characterized amplified region (SCAR), and random amplified polymorphic DNA (RAPD). All lines showed a chromosome number of 2n = 42, five of them carried both a pair of wheat-rye (Triticum aestivum-Secale cereal) 1BL/1RS translocation chromosomes and a pair of Agropyron intermedium (Ai) chromosomes, three carried a pair of Ai chromosomes only, three others carried a pair of 1BL/1RS chromosomes only, and one carried neither 1BL/1BS nor Ai chromosome. Further identification revealed that the identical Ai chromosome in these germplasm lines substituted the chromosome 2D of common wheat (T aestivum L.), designated as 2Ai. The genetic implication and further utilization of 2Ai in wheat improvement were also discussed.
关键词	Giant Spike Germplasm 1 Bl/1 Rs Agropyron Intermedium C-bandIng Genomic In Situ Hybridization (Gish) Sequence Characterized Amplified Region (Scar) Random Amplified Polymorphic Dna (Rapd)
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
学科领域	农作物
关键词[WOS]	INSITU HYBRIDIZATION ; TRITICUM-AESTIVUM ; TETRAPLOID WHEAT ; RYE CHROMATIN ; RESISTANCE ; MARKERS
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000186548600016
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/727
专题	中国科学院西北高原生物研究所
作者单位	1.Nanjing Agr Univ, Key Lab Crop Cytogenet, Minist Agr, Nanjing 210095, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Dou, QW,Chen, PD,Xie, JF. Cytological and molecular identification of alien chromatin in giant spike wheat germplasm[J]. ACTA BOTANICA SINICA,2003,45(9):1109-1115.
APA	Dou, QW,Chen, PD,&Xie, JF.(2003).Cytological and molecular identification of alien chromatin in giant spike wheat germplasm.ACTA BOTANICA SINICA,45(9),1109-1115.
MLA	Dou, QW,et al."Cytological and molecular identification of alien chromatin in giant spike wheat germplasm".ACTA BOTANICA SINICA 45.9(2003):1109-1115.

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