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Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography
Jiao, Lijin1,2; Tao, Yanduo1; Wang, Weidong1,2; Shao, Yun1; Mei, Lijuan1; Wang, Qilan1; Dang, Jun1
2017-10-01
Source PublicationJOURNAL OF SEPARATION SCIENCE
ISSN1615-9306
Volume40Issue:19Pages:3808-3816
SubtypeArticle
AbstractAn offline preparative two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography coupled with hydrophilic interaction solid-phase extraction method was developed for the preparative isolation of flavonoid glycosides from a crude sample of Sphaerophysa salsula. First, the non-flavonoids were removed using an XAmide solid-phase extraction cartridge. Based on the separation results of three different chromatographic stationary phases, the first-dimensional preparation was performed on an XAqua C18 prep column, and 15 fractions were obtained from the 5.2 g target sample. Then, three representative fractions were selected for additional purification on an XAmide preparative column to further isolate the flavonoid glycosides. In all, eight flavonoid glycosides were isolated in purities over 97%. The results demonstrated that the two-dimensional liquid chromatography method used in this study was effective for the preparative separation of flavonoid glycosides from Sphaerophysa salsula. Additionally, this method showed great potential for the separation of flavonoid glycosides from other plant materials.
KeywordFlavonoid Glycosides Hydrophilic Interaction Chromatography Hydrophilic Interaction Solid-phase Extraction Reversed-phase Liquid Chromatography Traditional Chinese Medicine
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1002/jssc.201700675
WOS KeywordCOUNTER-CURRENT CHROMATOGRAPHY ; FLIGHT MASS-SPECTROMETRY ; ASSISTED EXTRACTION ; PANAX-NOTOGINSENG ; MACROPOROUS RESIN ; SEPARATION ; LEAVES ; IDENTIFICATION ; PURIFICATION ; SAPONINS
Indexed BySCI
Language英语
Funding OrganizationProject of Discovery, Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Programme of Chinese Academy of Sciences(ZSTH-027) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017471) ; Significant Science & Technological Project of Qinghai Province(2014-GX-A3A)
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000412573400007
PublisherWILEY-V C H VERLAG GMBH
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/9809
Collection中国科学院西北高原生物研究所
Corresponding AuthorWang, Qilan; Dang, Jun
Affiliation1.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Jiao, Lijin,Tao, Yanduo,Wang, Weidong,et al. Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(19):3808-3816.
APA Jiao, Lijin.,Tao, Yanduo.,Wang, Weidong.,Shao, Yun.,Mei, Lijuan.,...&Dang, Jun.(2017).Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography.JOURNAL OF SEPARATION SCIENCE,40(19),3808-3816.
MLA Jiao, Lijin,et al."Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography".JOURNAL OF SEPARATION SCIENCE 40.19(2017):3808-3816.
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