Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography

doi:10.1002/jssc.201700675

NWIPB OpenIR

	Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography
	Jiao, Lijin 1,2; Tao, Yanduo 1; Wang, Weidong 1,2; Shao, Yun 1; Mei, Lijuan 1; Wang, Qilan1 ; Dang, Jun 1
	2017-10-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
ISSN	1615-9306
卷号	40 期号:19 页码:3808-3816
文章类型	Article
摘要	An offline preparative two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography coupled with hydrophilic interaction solid-phase extraction method was developed for the preparative isolation of flavonoid glycosides from a crude sample of Sphaerophysa salsula. First, the non-flavonoids were removed using an XAmide solid-phase extraction cartridge. Based on the separation results of three different chromatographic stationary phases, the first-dimensional preparation was performed on an XAqua C18 prep column, and 15 fractions were obtained from the 5.2 g target sample. Then, three representative fractions were selected for additional purification on an XAmide preparative column to further isolate the flavonoid glycosides. In all, eight flavonoid glycosides were isolated in purities over 97%. The results demonstrated that the two-dimensional liquid chromatography method used in this study was effective for the preparative separation of flavonoid glycosides from Sphaerophysa salsula. Additionally, this method showed great potential for the separation of flavonoid glycosides from other plant materials.
关键词	Flavonoid Glycosides Hydrophilic Interaction Chromatography Hydrophilic Interaction Solid-phase Extraction Reversed-phase Liquid Chromatography Traditional Chinese Medicine
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1002/jssc.201700675
关键词[WOS]	COUNTER-CURRENT CHROMATOGRAPHY ; FLIGHT MASS-SPECTROMETRY ; ASSISTED EXTRACTION ; PANAX-NOTOGINSENG ; MACROPOROUS RESIN ; SEPARATION ; LEAVES ; IDENTIFICATION ; PURIFICATION ; SAPONINS
收录类别	SCI
语种	英语
项目资助者	Project of Discovery, Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Programme of Chinese Academy of Sciences(ZSTH-027) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017471) ; Significant Science & Technological Project of Qinghai Province(2014-GX-A3A)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000412573400007
出版者	WILEY-V C H VERLAG GMBH
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/9809
专题	中国科学院西北高原生物研究所
通讯作者	Wang, Qilan; Dang, Jun
作者单位	1.Chinese Acad Sci, Key Lab Tibetan Med Res, Northwest Inst Plateau Biol, Xining, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Jiao, Lijin,Tao, Yanduo,Wang, Weidong,et al. Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(19):3808-3816.
APA	Jiao, Lijin.,Tao, Yanduo.,Wang, Weidong.,Shao, Yun.,Mei, Lijuan.,...&Dang, Jun.(2017).Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography.JOURNAL OF SEPARATION SCIENCE,40(19),3808-3816.
MLA	Jiao, Lijin,et al."Preparative isolation of flavonoid glycosides from Sphaerophysa salsula using hydrophilic interaction solid-phase extraction coupled with two-dimensional preparative liquid chromatography".JOURNAL OF SEPARATION SCIENCE 40.19(2017):3808-3816.