Efficient separation of high-purity compounds from Oxytropis falcata using two-dimensional preparative chromatography

doi:10.1002/jssc.201700449

NWIPB OpenIR

	Efficient separation of high-purity compounds from Oxytropis falcata using two-dimensional preparative chromatography
	Wang, Weidong 1,2; Tao, Yanduo 1; Jiao, Lijin 1,2; Fan, Minxia 1,2; Shao, Yun 1; Wang, Qilan1 ; Mei, Lijuan 1; Dang, Jun 1
	2017-09-01
发表期刊	JOURNAL OF SEPARATION SCIENCE
ISSN	1615-9306
卷号	40 期号:18 页码:3593-3601
文章类型	Article
摘要	The separation of high-purity compounds from traditional Tibetan medicines plays an important role in investigating their bioactivity. Nevertheless, it is often quite difficult to isolate compounds with high purity because of the complexity of traditional Tibetan medicines. In this work, an offline two-dimensional reversed-phase preparative method was successfully developed for the separation of high-purity compounds from Oxytropis falcata. Based on the analysis results, an ODS C18 prep column was used for first-dimensional preparation, and 14.8 g of the crude sample was separated into five fractions with a recovery of 74.6%. Then, an XAqua C18 prep column was used to isolate high-purity compounds in the second-dimensional preparation because its separation selectivity is different with the ODS C18 stationary phase. As a result, eight compounds in the crude sample were isolated in more than 98% purity. This is the first report of trans-cinnamic acid (1) and trifolirhizin (2) from Oxytropis falcata. This method has the potential to be an efficient separation method of high-purity compounds from Oxytropis falcata and it shows great promise for the separation of high-purity compounds from complex samples.
关键词	High-purity Compounds Oxytropis Falcata Traditional Tibetan Medicines Two-dimensional Liquid Chromatography
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1002/jssc.201700449
关键词[WOS]	PHASE LIQUID-CHROMATOGRAPHY ; ANTIOXIDANT ACTIVITIES ; BUNGE ; PURIFICATION ; FLAVONOIDS ; ENRICHMENT ; FRACTIONS ; EXTRACTS
收录类别	SCI
语种	英语
项目资助者	Project of Discovery, Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Programme of Chinese Academy of Sciences(ZSTH-027) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017471) ; Significant Science & Technological Project of Qinghai Province(2014-GX-A3A)
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000411740500003
出版者	WILEY-V C H VERLAG GMBH
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/9811
专题	中国科学院西北高原生物研究所
通讯作者	Mei, Lijuan; Dang, Jun
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Weidong,Tao, Yanduo,Jiao, Lijin,et al. Efficient separation of high-purity compounds from Oxytropis falcata using two-dimensional preparative chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(18):3593-3601.
APA	Wang, Weidong.,Tao, Yanduo.,Jiao, Lijin.,Fan, Minxia.,Shao, Yun.,...&Dang, Jun.(2017).Efficient separation of high-purity compounds from Oxytropis falcata using two-dimensional preparative chromatography.JOURNAL OF SEPARATION SCIENCE,40(18),3593-3601.
MLA	Wang, Weidong,et al."Efficient separation of high-purity compounds from Oxytropis falcata using two-dimensional preparative chromatography".JOURNAL OF SEPARATION SCIENCE 40.18(2017):3593-3601.