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Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles | |
Qu, Fei1,2; Liu, Yanqun1,2; Lao, Haili3; Wang, Yaping1,2; You, Jinmao1,2,4 | |
2017-10-07 | |
发表期刊 | NEW JOURNAL OF CHEMISTRY |
ISSN | 1144-0546 |
卷号 | 41期号:19页码:10592-10597 |
文章类型 | Article |
摘要 | Herein, a new colorimetric strategy based on polymer nanoparticles (PNPs) and Au nanoparticles (AuNPs) is developed to detect heparin (Hep). The cationic PNPs can directly induce the aggregation of AuNPs, which are stabilized by negatively charged coating of citrate ions, and hence the absorption peak of AuNPs moves to 675 nm corresponding with a color change from wine red to blue. However, the negatively charged Hep competes with AuNPs and binds to PNPs, hindering the interaction between PNPs and AuNPs. As a result, the absorption band of AuNPs blue shifts and the color turns to red again. The reaction can be completed within 10 min, and the color variation produced by 150 nM of Hep can be distinguished by the naked eye. In addition, this strategy also shows good selectivity and sensitivity, and the linear range of Hep is from 10 to 400 nM with detection limit of 2.5 nM. Finally, this colorimetric assay has been applied to measure Hep in Hep sodium injection and human serum samples with satisfactory recoveries. |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1039/c7nj02381b |
关键词[WOS] | PEROXIDASE-LIKE ACTIVITY ; INDUCED THROMBOCYTOPENIA ; THROMBOPLASTIN TIME ; INDUCED EMISSION ; QUANTUM DOTS ; SULFATE ; ASSAY ; NANOCOMPOSITES ; NANOCLUSTERS ; SURFACTANT |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China (Youth Fund Project)(21405093) ; Scientific Research Foundation of Qufu Normal University(BSQD20130117) |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Multidisciplinary |
WOS记录号 | WOS:000411768600011 |
出版者 | ROYAL SOC CHEMISTRY |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/9813 |
专题 | 中国科学院西北高原生物研究所 |
通讯作者 | Qu, Fei; You, Jinmao |
作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China 3.Binzhou Cent Hosp, Binzhou Med Coll, Dept Clin Lab, Binzhou 256600, Shandong, Peoples R China 4.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Qu, Fei,Liu, Yanqun,Lao, Haili,et al. Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles[J]. NEW JOURNAL OF CHEMISTRY,2017,41(19):10592-10597. |
APA | Qu, Fei,Liu, Yanqun,Lao, Haili,Wang, Yaping,&You, Jinmao.(2017).Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles.NEW JOURNAL OF CHEMISTRY,41(19),10592-10597. |
MLA | Qu, Fei,et al."Colorimetric detection of heparin with high sensitivity based on the aggregation of gold nanoparticles induced by polymer nanoparticles".NEW JOURNAL OF CHEMISTRY 41.19(2017):10592-10597. |
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