A sensitive pre-column derivatization method for the analysis of free fatty acids by RP-HPLC with fluorescence detector and its application to Caragana species

doi:10.1016/j.jchromb.2017.07.037

NWIPB OpenIR

	A sensitive pre-column derivatization method for the analysis of free fatty acids by RP-HPLC with fluorescence detector and its application to Caragana species
	Zeng, Zhi 1,3; Ji, Zhongyin 1,3; Hu, Na1 ; Bai, Bo 1,2; Wang, Honglun1,2 ; Suo, Yourui1,2
	2017-10-01
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
卷号	1064 页码:151-159
文章类型	Article
摘要	Caragana korshinskii Kom. (CK), one of afforestation tree species, is widely planted in northwest region of China. To compare the constituents as references for further utilization of CK, C. microphyll Lam. (CM) and C. jubata L. (CJ), been used as traditional Chinese medicine, were taken into consideration. To obtain more information on CK for further utilization, a sensitive and stable pre-column derivatization method for the analysis of fatty acids (FAs) was established using a novel labeling reagent 2-(5H-benzo[a]-carbazol-11(6H)-yl)ethyl hydrazine-carboxylate (BCEHC) by HPLC with fluorescence detector. The derivatives exhibit predominant fluorescence property at excitation and emission wavelengths of 330 urn and 380 nm, respectively. 16 derivatives of FAs including 13 saturated FAs and 3 unsaturated FAs are separated on a reversed-phase column with gradient elution within 30 min. The validation of method indicated that all FAs were given excellent linear responses with good linear coefficient of correlation being equal to or greater than 0.9985. The limits of detection (LODs) at a signal-to-noise ratio of 3 varied from 63.12 to 116.21 ng L-1. The developed method was successfully applied to determine the contents of free FAs (FFAs) in flowers, leaves and bark of CK and the samples were extracted by a green and simple method of gas purge microsyringe extraction. The results show that the contents of linoleic acid and linolenic acid are high, in flowers and leaves while the bark is rich in linoleic acid. The total content of FFAs in all parts of CK is higher than that of CM. The distribution of FFAs in plants is obviously different even in the congeneric among different species.
关键词	Caragana Korshinskii Kom. Hplc-fld 2-(5h-benzo[a]-carbazol-11(6h)-yl)Ethyl Hydrazine-carboxylate (Bcehc) Gas Purge Microsyringe Extraction Free Fatty Acid
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.jchromb.2017.07.037
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; PRESSURE CHEMICAL-IONIZATION ; MASS-SPECTROMETRY ; CARBOXYLIC-ACIDS ; GAS-CHROMATOGRAPHY ; SOIL PROPERTIES ; LOESS PLATEAU ; BILE-ACIDS ; SAMPLES ; CHINA
收录类别	SCI
语种	英语
项目资助者	Qinghai Science and Technology Department(2015-NK-302 ; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University(2017-KF-03) ; 2016-ZJ-Y01)
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000412958100021
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/9825
专题	中国科学院西北高原生物研究所
通讯作者	Bai, Bo
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Qinghai, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Zhi,Ji, Zhongyin,Hu, Na,et al. A sensitive pre-column derivatization method for the analysis of free fatty acids by RP-HPLC with fluorescence detector and its application to Caragana species[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1064:151-159.
APA	Zeng, Zhi,Ji, Zhongyin,Hu, Na,Bai, Bo,Wang, Honglun,&Suo, Yourui.(2017).A sensitive pre-column derivatization method for the analysis of free fatty acids by RP-HPLC with fluorescence detector and its application to Caragana species.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1064,151-159.
MLA	Zeng, Zhi,et al."A sensitive pre-column derivatization method for the analysis of free fatty acids by RP-HPLC with fluorescence detector and its application to Caragana species".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1064(2017):151-159.