NWIPB OpenIR
A sensitive pre-column derivatization method for the analysis of free fatty acids by RP-HPLC with fluorescence detector and its application to Caragana species
Zeng, Zhi1,3; Ji, Zhongyin1,3; Hu, Na1; Bai, Bo1,2; Wang, Honglun1,2; Suo, Yourui1,2
2017-10-01
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume1064Pages:151-159
SubtypeArticle
AbstractCaragana korshinskii Kom. (CK), one of afforestation tree species, is widely planted in northwest region of China. To compare the constituents as references for further utilization of CK, C. microphyll Lam. (CM) and C. jubata L. (CJ), been used as traditional Chinese medicine, were taken into consideration. To obtain more information on CK for further utilization, a sensitive and stable pre-column derivatization method for the analysis of fatty acids (FAs) was established using a novel labeling reagent 2-(5H-benzo[a]-carbazol-11(6H)-yl)ethyl hydrazine-carboxylate (BCEHC) by HPLC with fluorescence detector. The derivatives exhibit predominant fluorescence property at excitation and emission wavelengths of 330 urn and 380 nm, respectively. 16 derivatives of FAs including 13 saturated FAs and 3 unsaturated FAs are separated on a reversed-phase column with gradient elution within 30 min. The validation of method indicated that all FAs were given excellent linear responses with good linear coefficient of correlation being equal to or greater than 0.9985. The limits of detection (LODs) at a signal-to-noise ratio of 3 varied from 63.12 to 116.21 ng L-1. The developed method was successfully applied to determine the contents of free FAs (FFAs) in flowers, leaves and bark of CK and the samples were extracted by a green and simple method of gas purge microsyringe extraction. The results show that the contents of linoleic acid and linolenic acid are high, in flowers and leaves while the bark is rich in linoleic acid. The total content of FFAs in all parts of CK is higher than that of CM. The distribution of FFAs in plants is obviously different even in the congeneric among different species.
KeywordCaragana Korshinskii Kom. Hplc-fld 2-(5h-benzo[a]-carbazol-11(6h)-yl)Ethyl Hydrazine-carboxylate (Bcehc) Gas Purge Microsyringe Extraction Free Fatty Acid
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.jchromb.2017.07.037
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; PRESSURE CHEMICAL-IONIZATION ; MASS-SPECTROMETRY ; CARBOXYLIC-ACIDS ; GAS-CHROMATOGRAPHY ; SOIL PROPERTIES ; LOESS PLATEAU ; BILE-ACIDS ; SAMPLES ; CHINA
Indexed BySCI
Language英语
Funding OrganizationQinghai Science and Technology Department(2015-NK-302 ; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University(2017-KF-03) ; 2016-ZJ-Y01)
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000412958100021
PublisherELSEVIER SCIENCE BV
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/9825
Collection中国科学院西北高原生物研究所
Corresponding AuthorBai, Bo
Affiliation1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China
2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Qinghai, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zeng, Zhi,Ji, Zhongyin,Hu, Na,et al. A sensitive pre-column derivatization method for the analysis of free fatty acids by RP-HPLC with fluorescence detector and its application to Caragana species[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1064:151-159.
APA Zeng, Zhi,Ji, Zhongyin,Hu, Na,Bai, Bo,Wang, Honglun,&Suo, Yourui.(2017).A sensitive pre-column derivatization method for the analysis of free fatty acids by RP-HPLC with fluorescence detector and its application to Caragana species.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1064,151-159.
MLA Zeng, Zhi,et al."A sensitive pre-column derivatization method for the analysis of free fatty acids by RP-HPLC with fluorescence detector and its application to Caragana species".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1064(2017):151-159.
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