Knowledge Management System of Northwest Institute of Plateau Biology, CAS
A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine | |
Qu, Fei1,2; Liu, Yining1,2; Kong, Rongmei1,2; You, Jinmao1,2,3![]() | |
2017-11-01 | |
发表期刊 | MICROCHIMICA ACTA
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ISSN | 0026-3672 |
卷号 | 184期号:11页码:4417-4424 |
文章类型 | Article |
摘要 | The authors report that the fluorescence of polymer-capped silver nanoclusters (AgNCs) is quenched by MoS2 nanosheets. However, in the presence of electrolytes such as sodium acetate (50 mM), the nanosheets tend to aggregate, and this causes fluorescence to be restored. If, however, ssDNA is added, it will be adsorbed on the surface of the MoS2 nanosheets and stabilize them against aggregation. As a result, the emission of the AgNCs (best measured at excitation/emission wavelengths of 375/455 nm) is quenched again. If complementary oligonucleotide is added, a dsDNA will be formed that does no longer prevent aggregation and fluorescence is restored. Similarly, if an aptamer is adsorbed on the MoS2 nanosheets and its substrate is then added to form a folded state, the fluorescence of the AgNCs also will be restored. As a proof of concept, the DNA sequences of hepatitis B virus and of a dopamine-selective aptamer were chosen as models. The resulting assays have low detection limits and linear ranges that extend from 5 to 30 nM for hepatitis B DNA, and from 0.3 to 1 mu M for dopamine. In our perception, this method represents a universal platform for the detection of analytes for which complementary oligomers or aptamers are available. |
关键词 | Fluorescence Electrolytes Aggregation Ssdna Complementary Oligonucleotide Hepatitis b Virus Dopamine Dopamine-binding Aptamer |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1007/s00604-017-2486-7 |
关键词[WOS] | GOLD NANOPARTICLES ; LABEL-FREE ; ELECTRODE ; POLYETHYLENEIMINE ; BIOSENSOR ; RECOVERY ; PLATFORM ; IONS ; ACID ; DOTS |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | National Natural Science Foundation of China(21405093) ; Scientific Research Foundation of Qufu Normal University(BSQD20130117) |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000412901100023 |
出版者 | SPRINGER WIEN |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/9826 |
专题 | 中国科学院西北高原生物研究所 |
通讯作者 | Qu, Fei; You, Jinmao |
作者单位 | 1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China 2.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China |
推荐引用方式 GB/T 7714 | Qu, Fei,Liu, Yining,Kong, Rongmei,et al. A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine[J]. MICROCHIMICA ACTA,2017,184(11):4417-4424. |
APA | Qu, Fei,Liu, Yining,Kong, Rongmei,&You, Jinmao.(2017).A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine.MICROCHIMICA ACTA,184(11),4417-4424. |
MLA | Qu, Fei,et al."A versatile DNA detection scheme based on the quenching of fluorescent silver nanoclusters by MoS2 nanosheets: Application to aptamer-based determination of hepatitis B virus and of dopamine".MICROCHIMICA ACTA 184.11(2017):4417-4424. |
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