Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway | |
Zhao, Xiaoying2,3,4; Mei, Lijuan1; Pei, Jinjin1,5; Liu, Zenggen1; Shao, Yun1; Tao, Yanduo1; Zhang, Xiaoling2,3,4; Jiang, Lei1 | |
2017-11-08 | |
发表期刊 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY |
ISSN | 0021-8561 |
卷号 | 65期号:44页码:9647-9654 |
文章类型 | Article |
摘要 | An imbalance in osteogenesis and osteoclastogenesis is a crucial pathological factor in the development of osteoporosis. Osteoclasts (OCs) play a pivotal role in osteoporosis, whose new therapy exploration has been focused on the suppression of OC formation. Sophoridine is found from the Chinese traditional food sophora flower to exhibit anti-osteoporosis capacity by screening. This study is focused on its anti -osteoporosis mechanism evaluation. The anti -osteoporosis effect of sophoridine, (15 mg kg(-1) body), was evaluated in ovariectomized (OVX) mice by monitoring changes in bone histomorphometry index, formation of osteoclasts from blood-derived mononuclear cells, and changes in the synthesis of pro-osteodastogenic cytokines. Signal pathways were investigated by QPCR, Western blot, and immunofluorescence. Sophoridine has a significant anti-osteoporosis effect in vivo, which can inhibit RANKL-induced OC formation, the appearance of OC-specific marker genes, and OC marker protein in vitro. Mechanistically, sophoridine dose- and time-dependently blocks the RANKL-induced OC formation and the activation of ERK and c-Fos as well as the induction and nucleus translocation of NFATc1. Sophora flower might be a useful alternative functional food in preventing or treating osteoporosis. |
关键词 | Osteoporosis Sophora Flower Sophoridine Osteoclast Differentiation Mapk Nfatc1 |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1021/acs.jafc.7b03666 |
关键词[WOS] | BONE LOSS ; AGENTS |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Natural Science Foundation of Qinghai(2016-ZJ-942Q) ; West Light Foundation of the Chinese Academy of Sciences(Y629071211) ; National Natural Science Foundation of China(81572123 ; Science and Technology Commission of Shanghai Municipality(14431900900 ; Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support(20161314) ; 31701243) ; 115411951100 ; 116430723500) |
WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
WOS类目 | Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology |
WOS记录号 | WOS:000415028700010 |
出版者 | AMER CHEMICAL SOC |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/10360 |
专题 | 中国科学院西北高原生物研究所 |
通讯作者 | Zhang, Xiaoling; Jiang, Lei |
作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai 200025, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200025, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Med, Xin Hua Hosp, Dept Orthoped Surg, Shanghai 200092, Peoples R China 5.Shaanxi Univ Technol, Shaanxi Key Lab Bioresources & Biol, Hanzhong 723001, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, Xiaoying,Mei, Lijuan,Pei, Jinjin,et al. Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2017,65(44):9647-9654. |
APA | Zhao, Xiaoying.,Mei, Lijuan.,Pei, Jinjin.,Liu, Zenggen.,Shao, Yun.,...&Jiang, Lei.(2017).Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,65(44),9647-9654. |
MLA | Zhao, Xiaoying,et al."Sophoridine from Sophora Flower Attenuates Ovariectomy Induced Osteoporosis through the RANKL-ERK-NFAT Pathway".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 65.44(2017):9647-9654. |
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