Methanotrophic community structure and activity under warming and grazing of alpine meadow on the Tibetan Plateau

NWIPB OpenIR

	Methanotrophic community structure and activity under warming and grazing of alpine meadow on the Tibetan Plateau
	Zheng, Yong 1; Yang, Wei 1,3; Sun, Xiang 1; Wang, Shi-Ping 2; Rui, Yi-Chao 3; Luo, Cai-Yun 4; Guo, Liang-Dong 1
	2012-03-01
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN	0175-7598
卷号	93 期号:5 页码:2193-2203
文章类型	Article
摘要	Knowledge about methanotrophs and their activities is important to understand the microbial mediation of the greenhouse gas CH(4) under climate change and human activities in terrestrial ecosystems. The effects of simulated warming and sheep grazing on methanotrophic abundance, community composition, and activity were studied in an alpine meadow soil on the Tibetan Plateau. There was high abundance of methanotrophs (1.2-3.4？×？10(8) pmoA gene copies per gram of dry weight soil) assessed by real-time PCR, and warming significantly increased the abundance regardless of grazing. A total of 64 methanotrophic operational taxonomic units (OTUs) were obtained from 1,439 clone sequences, of these OTUs; 63 OTUs (98.4%) belonged to type I methanotrophs, and only one OTU was Methylocystis of type II methanotrophs. The methanotroph community composition and diversity were not apparently affected by the treatments. Warming and grazing significantly enhanced the potential CH(4) oxidation activity. There were significantly negative correlations between methanotrophic abundance and soil moisture and between methanotrophic abundance and NH(4)-N content. The study suggests that type I methanotrophs, as the dominance, may play a key role in CH(4) oxidation, and the alpine meadow has great potential to consume more CH(4) under future warmer and grazing conditions on the Tibetan Plateau.; Knowledge about methanotrophs and their activities is important to understand the microbial mediation of the greenhouse gas CH4 under climate change and human activities in terrestrial ecosystems. The effects of simulated warming and sheep grazing on methanotrophic abundance, community composition, and activity were studied in an alpine meadow soil on the Tibetan Plateau. There was high abundance of methanotrophs (1.2-3.4 x 10(8) pmoA gene copies per gram of dry weight soil) assessed by real-time PCR, and warming significantly increased the abundance regardless of grazing. A total of 64 methanotrophic operational taxonomic units (OTUs) were obtained from 1,439 clone sequences, of these OTUs; 63 OTUs (98.4%) belonged to type I methanotrophs, and only one OTU was Methylocystis of type II methanotrophs. The methanotroph community composition and diversity were not apparently affected by the treatments. Warming and grazing significantly enhanced the potential CH4 oxidation activity. There were significantly negative correlations between methanotrophic abundance and soil moisture and between methanotrophic abundance and NH4-N content. The study suggests that type I methanotrophs, as the dominance, may play a key role in CH4 oxidation, and the alpine meadow has great potential to consume more CH4 under future warmer and grazing conditions on the Tibetan Plateau.
关键词	Clone Library Grazing Methanotrophs Pmoa Tibetan Plateau Real-time
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	METHANE-OXIDIZING BACTERIA ; LANDFILL COVER SOIL ; 16S RIBOSOMAL-RNA ; MICROBIAL OXIDATION ; PLANT-COMMUNITIES ; GRASSLAND SOIL ; CLIMATE-CHANGE ; FOREST SOIL ; ECOSYSTEM ; TEMPERATURE
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000300657900036
引用统计	被引频次：78[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/2154
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, State Key Lab Mycol, Inst Microbiol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Tibetan Plateau Res, Key Lab Tibetan Environm Changes & Land Surface P, Beijing 100085, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 4.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Yong,Yang, Wei,Sun, Xiang,et al. Methanotrophic community structure and activity under warming and grazing of alpine meadow on the Tibetan Plateau[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2012,93(5):2193-2203.
APA	Zheng, Yong.,Yang, Wei.,Sun, Xiang.,Wang, Shi-Ping.,Rui, Yi-Chao.,...&Guo, Liang-Dong.(2012).Methanotrophic community structure and activity under warming and grazing of alpine meadow on the Tibetan Plateau.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,93(5),2193-2203.
MLA	Zheng, Yong,et al."Methanotrophic community structure and activity under warming and grazing of alpine meadow on the Tibetan Plateau".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 93.5(2012):2193-2203.

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