Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland

NWIPB OpenIR

	Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland
	Yang, Yunfeng ; Wu, Linwei ; Lin, Qiaoyan ; Yuan, Mengting ; Xu, Depeng ; Yu, Hao ; Hu, Yigang ; Duan, Jichuang ; Li, Xiangzhen ; He, Zhili ; Xue, Kai ; van Nostrand, Joy ; Wang, Shiping ; Zhou, Jizhong ; Yang, YF (reprint author), Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China.
	2013-02-01
发表期刊	GLOBAL CHANGE BIOLOGY ; Yang, YF; Wu, LW; Lin, QY; Yuan, MT; Xu, DP; Yu, H; Hu, YG; Duan, JC; Li, XZ; He, ZL; Xue, K; van Nostrand, J; Wang, SP; Zhou, JZ.Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland,GLOBAL CHANGE BIOLOGY,2013,19(2):637-648
摘要	Microbes play key roles in various biogeochemical processes, including carbon (C) and nitrogen (N) cycling. However, changes of microbial community at the functional gene level by livestock grazing, which is a global land-use activity, remain unclear. Here we use a functional gene array, GeoChip 4.0, to examine the effects of free livestock grazing on the microbial community at an experimental site of Tibet, a region known to be very sensitive to anthropogenic perturbation and global warming. Our results showed that grazing changed microbial community functional structure, in addition to aboveground vegetation and soil geochemical properties. Further statistical tests showed that microbial community functional structures were closely correlated with environmental variables, and variations in microbial community functional structures were mainly controlled by aboveground vegetation, soil C/N ratio, and NH4+-N. In-depth examination of N cycling genes showed that abundances of N mineralization and nitrification genes were increased at grazed sites, but denitrification and N-reduction genes were decreased, suggesting that functional potentials of relevant bioprocesses were changed. Meanwhile, abundances of genes involved in methane cycling, C fixation, and degradation were decreased, which might be caused by vegetation removal and hence decrease in litter accumulation at grazed sites. In contrast, abundances of virulence, stress, and antibiotics resistance genes were increased because of the presence of livestock. In conclusion, these results indicated that soil microbial community functional structure was very sensitive to the impact of livestock grazing and revealed microbial functional potentials in regulating soil N and C cycling, supporting the necessity to include microbial components in evaluating the consequence of land-use and/or climate changes.; Microbes play key roles in various biogeochemical processes, including carbon (C) and nitrogen (N) cycling. However, changes of microbial community at the functional gene level by livestock grazing, which is a global land-use activity, remain unclear. Here we use a functional gene array, GeoChip 4.0, to examine the effects of free livestock grazing on the microbial community at an experimental site of Tibet, a region known to be very sensitive to anthropogenic perturbation and global warming. Our results showed that grazing changed microbial community functional structure, in addition to aboveground vegetation and soil geochemical properties. Further statistical tests showed that microbial community functional structures were closely correlated with environmental variables, and variations in microbial community functional structures were mainly controlled by aboveground vegetation, soil C/N ratio, and NH4+-N. In-depth examination of N cycling genes showed that abundances of N mineralization and nitrification genes were increased at grazed sites, but denitrification and N-reduction genes were decreased, suggesting that functional potentials of relevant bioprocesses were changed. Meanwhile, abundances of genes involved in methane cycling, C fixation, and degradation were decreased, which might be caused by vegetation removal and hence decrease in litter accumulation at grazed sites. In contrast, abundances of virulence, stress, and antibiotics resistance genes were increased because of the presence of livestock. In conclusion, these results indicated that soil microbial community functional structure was very sensitive to the impact of livestock grazing and revealed microbial functional potentials in regulating soil N and C cycling, supporting the necessity to include microbial components in evaluating the consequence of land-use and/or climate changes.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/26930
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yang, Yunfeng,Wu, Linwei,Lin, Qiaoyan,et al. Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland[J]. GLOBAL CHANGE BIOLOGY, Yang, YF; Wu, LW; Lin, QY; Yuan, MT; Xu, DP; Yu, H; Hu, YG; Duan, JC; Li, XZ; He, ZL; Xue, K; van Nostrand, J; Wang, SP; Zhou, JZ.Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland,GLOBAL CHANGE BIOLOGY,2013,19(2):637-648,2013.
APA	Yang, Yunfeng.,Wu, Linwei.,Lin, Qiaoyan.,Yuan, Mengting.,Xu, Depeng.,...&Yang, YF .(2013).Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland.GLOBAL CHANGE BIOLOGY.
MLA	Yang, Yunfeng,et al."Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland".GLOBAL CHANGE BIOLOGY (2013).