Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling

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	Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling
	Song, Cuihua ; Zhang, Caiqing ; Li, Guoliang ; Zhang, Xiaolong ; Chen, Guang ; You, Jinmao
	2012-03-01
发表期刊	INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION ; Qu, JP; Liu, M; Yang, M; Zhang, YM; Ji, WH.Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling,INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION,2012,63(2):170-177
摘要	Amino acids (AA) are important chemical constituents of tea leaves remarkably influencing the quality of tea. In this study, free AA and total AA in Apocynum venetum L. (Luobuma tea) were estimated by HPLC equipped with fluorescent detector using 2-[2-(7H-dibenzo[a,g]carbazol-7-yl)-ethoxy] ethyl chloroformate (DBCEEC) as a fluorescent labelling reagent. Different parameters for derivatization and separation were optimized. AA were rapidly derivatized within 3 min at room temperature with DBCEEC. In conjunction with a gradient elution, a baseline resolution of 20 analytes was achieved on a reversed-phase Hypersil BDS C18 column. LC separation for the derivatized AA showed good reproducibility. Twenty AA were detected and showed significant linear responses with correlation coefficients (>0.9992). This developed method offered the low detection limit of 2.88-23.4 fmol.; Amino acids (AA) are important chemical constituents of tea leaves remarkably influencing the quality of tea. In this study, free AA and total AA in Apocynum venetum L. (Luobuma tea) were estimated by HPLC equipped with fluorescent detector using 2-[2-(7H-dibenzo[a,g]carbazol-7-yl)-ethoxy] ethyl chloroformate (DBCEEC) as a fluorescent labelling reagent. Different parameters for derivatization and separation were optimized. AA were rapidly derivatized within 3 min at room temperature with DBCEEC. In conjunction with a gradient elution, a baseline resolution of 20 analytes was achieved on a reversed-phase Hypersil BDS C18 column. LC separation for the derivatized AA showed good reproducibility. Twenty AA were detected and showed significant linear responses with correlation coefficients (>0.9992). This developed method offered the low detection limit of 2.88-23.4 fmol.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/31805
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Song, Cuihua,Zhang, Caiqing,Li, Guoliang,et al. Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling[J]. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, Qu, JP; Liu, M; Yang, M; Zhang, YM; Ji, WH.Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling,INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION,2012,63(2):170-177,2012.
APA	Song, Cuihua,Zhang, Caiqing,Li, Guoliang,Zhang, Xiaolong,Chen, Guang,&You, Jinmao.(2012).Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling.INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION.
MLA	Song, Cuihua,et al."Highly selective and sensitive determination of free and total amino acids in Apocynum venetum L. (Luobuma tea) by a developed HPLC-FLD method coupled with pre-column fluorescent labelling".INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION (2012).