NWIPB OpenIR
An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier
Huang, Qiong ; Bu, Tong ; Zhang, Wentao ; Yan, Lingzhi ; Zhang, Mengyue ; Yang, Qingfeng ; Huang, Lunjie ; Yang, Baowei ; Hu, Na ; Suo, Yourui ; Wang, Jianlong ; Zhang, Daohong
2018-10-01
Source PublicationFOOD CHEMISTRY
AbstractImmunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1 ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors.
PublisherELSEVIER SCI LTD
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/39315
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Huang, Qiong,Bu, Tong,Zhang, Wentao,et al. An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier[J]. FOOD CHEMISTRY,2018.
APA Huang, Qiong.,Bu, Tong.,Zhang, Wentao.,Yan, Lingzhi.,Zhang, Mengyue.,...&Zhang, Daohong.(2018).An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier.FOOD CHEMISTRY.
MLA Huang, Qiong,et al."An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier".FOOD CHEMISTRY (2018).
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