NWIPB OpenIR
An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier
Huang, Qiong ; Bu, Tong ; Zhang, Wentao ; Yan, Lingzhi ; Zhang, Mengyue ; Yang, Qingfeng ; Huang, Lunjie ; Yang, Baowei ; Hu, Na ; Suo, Yourui ; Wang, Jianlong ; Zhang, Daohong
2018-10-01
发表期刊FOOD CHEMISTRY
摘要Immunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1 ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors.
出版者ELSEVIER SCI LTD
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/39315
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Huang, Qiong,Bu, Tong,Zhang, Wentao,et al. An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier[J]. FOOD CHEMISTRY,2018.
APA Huang, Qiong.,Bu, Tong.,Zhang, Wentao.,Yan, Lingzhi.,Zhang, Mengyue.,...&Zhang, Daohong.(2018).An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier.FOOD CHEMISTRY.
MLA Huang, Qiong,et al."An improved clenbuterol detection by immunochromatographic assay with bacteria@Au composite as signal amplifier".FOOD CHEMISTRY (2018).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Huang, Qiong]的文章
[Bu, Tong]的文章
[Zhang, Wentao]的文章
百度学术
百度学术中相似的文章
[Huang, Qiong]的文章
[Bu, Tong]的文章
[Zhang, Wentao]的文章
必应学术
必应学术中相似的文章
[Huang, Qiong]的文章
[Bu, Tong]的文章
[Zhang, Wentao]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。