Determination of thiols by gas purge microsyringe extraction coupled with chemical derivatization by high performance liquid chromatography-fluorescence detection with mass spectrometry identification

NWIPB OpenIR

	Determination of thiols by gas purge microsyringe extraction coupled with chemical derivatization by high performance liquid chromatography-fluorescence detection with mass spectrometry identification
	Zhuang, Jianlin; You, Jinmao; Zhang, Shijuan; Sun, Zhiwei; Ji, Zhongyin; Liu, Jiamin; Yu, Yanxin
	2018
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
卷号	41 期号:12
摘要	A new fluorescence labeling reagent 1-(4-(1H-phenanthro[9,10-d]imidazol-2-yl)phenyl)-1H-pyrrole-5-dione (PIPD) used for the sensitive determination of thiols in food samples has been successfully prepared. Thiols were extracted by a gas purge microsyringe extraction (GP-MSE) device. The derivatization of PIPD with thiols could be finished within 10minutes at 35 degrees C in weak alkaline condition, and the corresponding derivatives showed excellent fluorescence properties with excitation and emission wavelengths of 264nm and 396nm, respectively. The eight thiol derivatives could be easily separated on normal reversed phase column because of the introduction of highly hydrophobic PIPD unit into the target analytes. It is the first time that GP-MSE has been applied to the extraction of a wide range of thiols in food samples with only a small amount of methanol (0.5mL) needed. The limits of detection (LODs) were in the range of 0.19-0.98 mu g/kg, while the recoveries were in the range of 85.6%-97.1% with relative standard deviations (RSDs)<4.98%. Good linearities were observed for all analytes with correlation coefficients of higher than>0.9995. [GRAPHICS] .
关键词	Derivatization food sample GP-MSE HPLC-MS PIPD thiols
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/60024
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Zhuang, Jianlin,You, Jinmao,Zhang, Shijuan,et al. Determination of thiols by gas purge microsyringe extraction coupled with chemical derivatization by high performance liquid chromatography-fluorescence detection with mass spectrometry identification[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2018,41(12).
APA	Zhuang, Jianlin.,You, Jinmao.,Zhang, Shijuan.,Sun, Zhiwei.,Ji, Zhongyin.,...&Yu, Yanxin.(2018).Determination of thiols by gas purge microsyringe extraction coupled with chemical derivatization by high performance liquid chromatography-fluorescence detection with mass spectrometry identification.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,41(12).
MLA	Zhuang, Jianlin,et al."Determination of thiols by gas purge microsyringe extraction coupled with chemical derivatization by high performance liquid chromatography-fluorescence detection with mass spectrometry identification".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 41.12(2018).