Changes of hemoglobin expression in response to hypoxia in a Tibetan schizothoracine fish, Schizopygopsis pylzovi

doi:10.1007/s00360-016-1013-1

NWIPB OpenIR

	Changes of hemoglobin expression in response to hypoxia in a Tibetan schizothoracine fish, Schizopygopsis pylzovi
	Xia, Mingzhe 1,2,3; Chao, Yan 1,3; Jia, Jianlei 3; Li, Changzhong 1,2; Kong, Qinghui 3; Zhao, Yongli 1,2,3; Guo, Songchang 4; Qi, Delin 1,2
	2016-12-01
发表期刊	JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY
卷号	186 期号:8 页码:1033-1043
文章类型	Article
摘要	Fishes endemic to the Qinghai-Tibetan Plateau are comparatively well adapted to aquatic environments with low oxygen partial pressures (hypoxia). Here, we cloned the complete cDNA of hemoglobin (Hb) alpha and beta from the Tibetan schizothoracine fish Schizopygopsis pylzovi, and then investigated changes in Hb mRNA and protein levels in spleen, liver and kidney in response to hypoxia. We applied severe hypoxia (4 h at PO2 = 0.6 kPa) and moderate hypoxia (72 h at PO2 = 6.0 kPa) to adult S. pylzovi. Changes of Hb expression under hypoxia, together with the investigations of spleen somatic index, kidney somatic index and Hb concentration in circulation, suggest that the kidney may not only serve as the erythropoietic organ, but also act as the major blood reservoir in S. pylzovi. From this perspective, the transcriptional activity of Hb in S. pylzovi, as reflected in the kidney, was turned down quickly after the onset of severe hypoxia, while under moderate hypoxia the transcriptional activity of Hb showed upregulation for a short time, but then the transcriptional machinery was turned down slowly on prolonged exposure. Notably, the changes in Hb protein levels in spleen, liver and kidney in response to severe and moderate hypoxia were not in line with the changes in mRNA levels, which are related with the blood reservoir in the kidney. Tibetan schizothoracine fish, at least S. pylzovi, show a particular response of the transcription regulation of Hb to moderate hypoxia, which is different from that of other fish species.
关键词	Hemoglobin Hypoxia Expression Regulation Hb Concentration Schizopygopsis Pylzovi Qinghai-tibetan Plateau
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00360-016-1013-1
关键词[WOS]	GOLDFISH CARASSIUS-AURATUS ; GENE-EXPRESSION ; LYMPHOID ORGANS ; AQUATIC HYPOXIA ; TEMPERATURE ; CARP ; ZEBRAFISH ; FLOUNDER ; GILL ; ERYTHROCYTES
收录类别	SCI
语种	英语
项目资助者	National Natural Science Foundation of China(31460094) ; Natural Science Foundation of Qinghai Science & Technology Department in China(2015-ZJ-901)
WOS研究方向	Physiology ; Zoology
WOS类目	Physiology ; Zoology
WOS记录号	WOS:000387350300007
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/6672
专题	中国科学院西北高原生物研究所
作者单位	1.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Peoples R China 2.Qinghai Univ, Coll Ecoenvironm Engn, Xining 810016, Peoples R China 3.Qinghai Univ, Dept Anim Sci, Agr & Anim Husb Coll, Xining 810016, Peoples R China 4.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Xia, Mingzhe,Chao, Yan,Jia, Jianlei,et al. Changes of hemoglobin expression in response to hypoxia in a Tibetan schizothoracine fish, Schizopygopsis pylzovi[J]. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,2016,186(8):1033-1043.
APA	Xia, Mingzhe.,Chao, Yan.,Jia, Jianlei.,Li, Changzhong.,Kong, Qinghui.,...&Qi, Delin.(2016).Changes of hemoglobin expression in response to hypoxia in a Tibetan schizothoracine fish, Schizopygopsis pylzovi.JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY,186(8),1033-1043.
MLA	Xia, Mingzhe,et al."Changes of hemoglobin expression in response to hypoxia in a Tibetan schizothoracine fish, Schizopygopsis pylzovi".JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY 186.8(2016):1033-1043.