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Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology
Zeng, Zhi1,3; Ji, Zhongyin1,3; Hu, Na1; Chen, Shasha1,3; Bai, Bo1,2; Wang, Honglun1,2; Suo, Yourui1,2
2017-06-05
发表期刊JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷号140页码:182-189
文章类型Article
摘要The utilization of Caragana korshinskii Kom. (CK) is currently concentrated on its ecological and fuel functions. Little attention has been devoted to the analysis of their phenolic acid (PA) components. To obtain more data for further utilization of CK, a new analysis protocol was tested to determine PAs synchronously by RP-HPLC-UV with double-wavelength (280 nm and 320 nm) detection. Specifically, separation of PA components was performed on a Hypersil Gold C-18 reverse phase column with gradient elution. A four factor-three-level Box-Behnken design was implemented for optimization of PA extraction. The results demonstrated that CK were rich primarily in chlorogenic acid, vanillic acid, caffeic acid and rosmarinic acid. The total content of PAs in CK leaves was the highest compared with its other parts. The distribution of total flavonoid content of CK was leaves > flowers > bark, while that of the total phenolic content of CK was flowers > leaves > bark. (C) 2017 Elsevier B.V. All rights reserved.
关键词Response Surface Methodology (Rsm) Rp-hplc-uv Phenolic Acids Caragana Korshinskii Kom
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
DOI10.1016/j.jpba.2017.03.017
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; ANTIOXIDANT ACTIVITY ; FLUORESCENCE DETECTION ; FATTY-ACIDS ; OLIVE OIL ; CONSTITUENTS ; FLAVONOIDS ; BERRIES ; APPLES ; LEAVES
收录类别SCI
语种英语
项目资助者Spark Program of Qinghai Science and Technology Department(2015-NK-302) ; Project of Qinghai Science and Technology Department(2016-ZJ-Y01) ; Open Project of State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University(2017-KF-03)
WOS研究方向Chemistry ; Pharmacology & Pharmacy
WOS类目Chemistry, Analytical ; Pharmacology & Pharmacy
WOS记录号WOS:000402850500022
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/7014
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China
2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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GB/T 7714
Zeng, Zhi,Ji, Zhongyin,Hu, Na,et al. Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017,140:182-189.
APA Zeng, Zhi.,Ji, Zhongyin.,Hu, Na.,Chen, Shasha.,Bai, Bo.,...&Suo, Yourui.(2017).Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,140,182-189.
MLA Zeng, Zhi,et al."Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 140(2017):182-189.
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