Knowledge Management System of Northwest Institute of Plateau Biology, CAS
Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology | |
Zeng, Zhi1,3; Ji, Zhongyin1,3; Hu, Na1; Chen, Shasha1,3; Bai, Bo1,2; Wang, Honglun1,2; Suo, Yourui1,2 | |
2017-06-05 | |
发表期刊 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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卷号 | 140页码:182-189 |
文章类型 | Article |
摘要 | The utilization of Caragana korshinskii Kom. (CK) is currently concentrated on its ecological and fuel functions. Little attention has been devoted to the analysis of their phenolic acid (PA) components. To obtain more data for further utilization of CK, a new analysis protocol was tested to determine PAs synchronously by RP-HPLC-UV with double-wavelength (280 nm and 320 nm) detection. Specifically, separation of PA components was performed on a Hypersil Gold C-18 reverse phase column with gradient elution. A four factor-three-level Box-Behnken design was implemented for optimization of PA extraction. The results demonstrated that CK were rich primarily in chlorogenic acid, vanillic acid, caffeic acid and rosmarinic acid. The total content of PAs in CK leaves was the highest compared with its other parts. The distribution of total flavonoid content of CK was leaves > flowers > bark, while that of the total phenolic content of CK was flowers > leaves > bark. (C) 2017 Elsevier B.V. All rights reserved. |
关键词 | Response Surface Methodology (Rsm) Rp-hplc-uv Phenolic Acids Caragana Korshinskii Kom |
WOS标题词 | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine |
DOI | 10.1016/j.jpba.2017.03.017 |
关键词[WOS] | PERFORMANCE LIQUID-CHROMATOGRAPHY ; ANTIOXIDANT ACTIVITY ; FLUORESCENCE DETECTION ; FATTY-ACIDS ; OLIVE OIL ; CONSTITUENTS ; FLAVONOIDS ; BERRIES ; APPLES ; LEAVES |
收录类别 | SCI |
语种 | 英语 |
项目资助者 | Spark Program of Qinghai Science and Technology Department(2015-NK-302) ; Project of Qinghai Science and Technology Department(2016-ZJ-Y01) ; Open Project of State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University(2017-KF-03) |
WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
WOS类目 | Chemistry, Analytical ; Pharmacology & Pharmacy |
WOS记录号 | WOS:000402850500022 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/7014 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China 2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Zeng, Zhi,Ji, Zhongyin,Hu, Na,et al. Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017,140:182-189. |
APA | Zeng, Zhi.,Ji, Zhongyin.,Hu, Na.,Chen, Shasha.,Bai, Bo.,...&Suo, Yourui.(2017).Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,140,182-189. |
MLA | Zeng, Zhi,et al."Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 140(2017):182-189. |
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