NWIPB OpenIR
Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology
Zeng, Zhi1,3; Ji, Zhongyin1,3; Hu, Na1; Chen, Shasha1,3; Bai, Bo1,2; Wang, Honglun1,2; Suo, Yourui1,2
2017-06-05
Source PublicationJOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume140Pages:182-189
SubtypeArticle
AbstractThe utilization of Caragana korshinskii Kom. (CK) is currently concentrated on its ecological and fuel functions. Little attention has been devoted to the analysis of their phenolic acid (PA) components. To obtain more data for further utilization of CK, a new analysis protocol was tested to determine PAs synchronously by RP-HPLC-UV with double-wavelength (280 nm and 320 nm) detection. Specifically, separation of PA components was performed on a Hypersil Gold C-18 reverse phase column with gradient elution. A four factor-three-level Box-Behnken design was implemented for optimization of PA extraction. The results demonstrated that CK were rich primarily in chlorogenic acid, vanillic acid, caffeic acid and rosmarinic acid. The total content of PAs in CK leaves was the highest compared with its other parts. The distribution of total flavonoid content of CK was leaves > flowers > bark, while that of the total phenolic content of CK was flowers > leaves > bark. (C) 2017 Elsevier B.V. All rights reserved.
KeywordResponse Surface Methodology (Rsm) Rp-hplc-uv Phenolic Acids Caragana Korshinskii Kom
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
DOI10.1016/j.jpba.2017.03.017
WOS KeywordPERFORMANCE LIQUID-CHROMATOGRAPHY ; ANTIOXIDANT ACTIVITY ; FLUORESCENCE DETECTION ; FATTY-ACIDS ; OLIVE OIL ; CONSTITUENTS ; FLAVONOIDS ; BERRIES ; APPLES ; LEAVES
Indexed BySCI
Language英语
Funding OrganizationSpark Program of Qinghai Science and Technology Department(2015-NK-302) ; Project of Qinghai Science and Technology Department(2016-ZJ-Y01) ; Open Project of State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University(2017-KF-03)
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000402850500022
Citation statistics
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/7014
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China
2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zeng, Zhi,Ji, Zhongyin,Hu, Na,et al. Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017,140:182-189.
APA Zeng, Zhi.,Ji, Zhongyin.,Hu, Na.,Chen, Shasha.,Bai, Bo.,...&Suo, Yourui.(2017).Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,140,182-189.
MLA Zeng, Zhi,et al."Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 140(2017):182-189.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zeng, Zhi]'s Articles
[Ji, Zhongyin]'s Articles
[Hu, Na]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zeng, Zhi]'s Articles
[Ji, Zhongyin]'s Articles
[Hu, Na]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zeng, Zhi]'s Articles
[Ji, Zhongyin]'s Articles
[Hu, Na]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.