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A developed pre-column derivatization method for the determination of free fatty acids in edible oils by reversed-phase HPLC with fluorescence detection and its application to Lycium barbarum seed oil
Li, Guoliang1,2,3; You, Jinmao1,2; Suo, Yourui1; Song, Cuihua2; Sun, Zhiwei1,3; Xia, Lian2; Zhao, Xianen4; Shi, Junyou1,3
2011-04-14
Source PublicationFOOD CHEMISTRY
ISSN0308-8146
Volume125Issue:4Pages:1365-1372
SubtypeArticle
AbstractFree fatty acids (FFAs) in edible oils are one of the most frequently determined quality indices during production storage and marketing In this study a simple stable and sensitive method for the simultaneous determination of saturated and unsaturated FFAs from edible oils using 2-(11H benzo[a]carbazol-11-yl)-ethyl-4-methylbenzenesulphonate (BCETS) as labelling reagent with fluorescence detection has been established FFAs are derivatized by BCETS and separated on a reversed-phase Eclipse XDB-C(8) column with a gradient elution The results indicated that all FFAs were found to be given an excellent linear response with correlation coefficients of >0 9994 The detection limits at a signal-to noise ratio of 3 were in the range of 0 22-1 06 ng/mL. When applied to Lycium barbarum seed oils the developed method showed good reproducibility The effect of extraction methods including supercritical CO(2) and organic solvent extraction on the FFAs composition has also been investigated Meanwhile this method exhibits powerful potential for the trace analysis of short- and long chain free fatty acids from edible oils food-stuff and other complex samples (C) 2010 Elsevier Ltd All rights reserved; Free fatty acids (FFAs) in edible oils are one of the most frequently determined quality indices during production storage and marketing In this study a simple stable and sensitive method for the simultaneous determination of saturated and unsaturated FFAs from edible oils using 2-(11H benzo[a]carbazol-11-yl)-ethyl-4-methylbenzenesulphonate (BCETS) as labelling reagent with fluorescence detection has been established FFAs are derivatized by BCETS and separated on a reversed-phase Eclipse XDB-C(8) column with a gradient elution The results indicated that all FFAs were found to be given an excellent linear response with correlation coefficients of >0 9994 The detection limits at a signal-to noise ratio of 3 were in the range of 0 22-1 06 ng/mL. When applied to Lycium barbarum seed oils the developed method showed good reproducibility The effect of extraction methods including supercritical CO(2) and organic solvent extraction on the FFAs composition has also been investigated Meanwhile this method exhibits powerful potential for the trace analysis of short- and long chain free fatty acids from edible oils food-stuff and other complex samples (C) 2010 Elsevier Ltd All rights reserved
KeywordFree Fatty Acids Edible Oil Lycium Barbarum l Derivatization Hplc-ms/ms
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine
WOS KeywordTRANSFORM INFRARED-SPECTROSCOPY ; CRUDE PALM OIL ; FLOW-INJECTION ; OXIDATIVE STRESS ; OLIVE OIL ; POLYSACCHARIDES ; IDENTIFICATION ; EXTRACTION ; TITRATION ; ALCOHOLS
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS SubjectChemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS IDWOS:000285444900038
Citation statistics
Cited Times:39[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1556
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
2.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan 250014, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Li, Guoliang,You, Jinmao,Suo, Yourui,et al. A developed pre-column derivatization method for the determination of free fatty acids in edible oils by reversed-phase HPLC with fluorescence detection and its application to Lycium barbarum seed oil[J]. FOOD CHEMISTRY,2011,125(4):1365-1372.
APA Li, Guoliang.,You, Jinmao.,Suo, Yourui.,Song, Cuihua.,Sun, Zhiwei.,...&Shi, Junyou.(2011).A developed pre-column derivatization method for the determination of free fatty acids in edible oils by reversed-phase HPLC with fluorescence detection and its application to Lycium barbarum seed oil.FOOD CHEMISTRY,125(4),1365-1372.
MLA Li, Guoliang,et al."A developed pre-column derivatization method for the determination of free fatty acids in edible oils by reversed-phase HPLC with fluorescence detection and its application to Lycium barbarum seed oil".FOOD CHEMISTRY 125.4(2011):1365-1372.
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