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Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity
Liu, Zenggen1,2; Dang, Jun1,2; Wang, Qilan1; Yu, Minfeng1,2; Jiang, Lei1,2; Mei, Lijuan1; Shao, Yun1; Tao, Yanduo1
2013-10-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume61Pages:127-134
SubtypeArticle
AbstractDynamic microwave-assisted extraction (DMAE) technique was employed for the extraction of polysaccharides from Lycium ruthenicum (LRP). The extracting parameters were optimized by using three-variable-three-level Box-Behnken design and response surface methodology (RSM) based on the single-factor experiments. RSM analysis indicated good correspondence between experimental and predicted values. The optimum extraction parameters for the yield of polysaccharide were ratio of water to raw material 31.5 mL/g, extracting time 25.8 min and microwave power 544.0W. Polysaccharide was analyzed by chemical methods and Fourier-transform infrared (FT-IR). The antioxidant activities of LRP were investigated including scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide and free radicals of superoxide anion in vitro. The results of antioxidant activity exhibited LRP had the potential to be explored as novel natural antioxidant for using in functional foods or medicine. (C) 2013 Elsevier B.V. All rights reserved.
KeywordLycium Ruthenicum Murr. Polysaccharides Antioxidant Activity
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.ijbiomac.2013.06.042
WOS KeywordMICROWAVE-ASSISTED EXTRACTION ; RESPONSE-SURFACE METHODOLOGY ; STRUCTURAL-CHARACTERIZATION ; SUPEROXIDE-DISMUTASE ; IN-VITRO ; PURIFICATION ; AUTOXIDATION ; DERIVATIVES ; BARBARUM ; PROTEIN
Indexed BySCI
Language英语
Funding OrganizationMinistry of Science and Technology of PR China(2007BAI45B00)
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000326660700019
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/3914
Collection中国科学院西北高原生物研究所
Corresponding AuthorShao, Yun
Affiliation1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810001, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Liu, Zenggen,Dang, Jun,Wang, Qilan,et al. Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2013,61:127-134.
APA Liu, Zenggen.,Dang, Jun.,Wang, Qilan.,Yu, Minfeng.,Jiang, Lei.,...&Tao, Yanduo.(2013).Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,61,127-134.
MLA Liu, Zenggen,et al."Optimization of polysaccharides from Lycium ruthenicum fruit using RSM and its anti-oxidant activity".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 61(2013):127-134.
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