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Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity
Zhang, Gong ; Chen, Shasha ; Zhou, Wu ; Meng, Jing ; Deng, Kai ; Zhou, Haonan ; Hu, Na ; Suo, Yourui
2018-12-15
Source PublicationFOOD CHEMISTRY
AbstractLycium ruthenicum Murray (LR) is a functional food, and it has long been used in traditional folk medicine. However, detailed qualitative and quantitative analyses related to its phenolic compounds remains scarce. This work reports, for the first time, the establishment of a rapid method for simultaneous identification and quantification of 25 phenolic compounds by UPLC-quadrupole-Orbitrap mass spectrometry (UPLC-Q-Orbitrap MS). This method was validated by LODs, LOQs, precision, repeatability, stability, mean recovery, recovery range and RSD. The confirmed method was applied to the analysis of phenolic compounds in LR. Finally, 18 phenolic compounds in LR were qualitatively and quantitatively analyzed. Among them, 11 constituents were detected for the first time, which included two flavonoids (catechin and naringenin) and seven phenolic acids (gallic acid, vanillic acid, 2,4-dihydroxybenzoic acid, veratronic acid, benzoic acid, ellagic acid and salicylic acid). Moreover, Phloretin and protocatechuate, belonging to the dihydrochalcone flavonoid and protocatechuic acid respectively, were also identified and quantified. The total phenolics content (20.17 +/- 2.82 mg/g) and the total anthocyanin content (147.43 +/- 1.81 mg/g) were determined. In addition, the antioxidant activities of the LR extract were evaluated through 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP) and total antioxidant activity (T-AOC) assays.
PublisherELSEVIER SCI LTD
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/39296
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Zhang, Gong,Chen, Shasha,Zhou, Wu,et al. Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity[J]. FOOD CHEMISTRY,2018.
APA Zhang, Gong.,Chen, Shasha.,Zhou, Wu.,Meng, Jing.,Deng, Kai.,...&Suo, Yourui.(2018).Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity.FOOD CHEMISTRY.
MLA Zhang, Gong,et al."Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity".FOOD CHEMISTRY (2018).
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