Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity

doi:10.1016/j.foodchem.2018.06.132

NWIPB OpenIR

	Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity
	Zhang, Gong 1,2,4,5; Chen, Shasha 1,4,5; Zhou, Wu 1,3,4,5; Meng, Jing 1,4,5; Deng, Kai 1,4,5; Zhou, Haonan 1,4,5; Hu, Na 1,4; Suo, Yourui1,3,4
	2018-12-15
发表期刊	FOOD CHEMISTRY
ISSN	0308-8146
卷号	269 页码:150-156
文章类型	Article
摘要	Lycium ruthenicum Murray (LR) is a functional food, and it has long been used in traditional folk medicine. However, detailed qualitative and quantitative analyses related to its phenolic compounds remains scarce. This work reports, for the first time, the establishment of a rapid method for simultaneous identification and quantification of 25 phenolic compounds by UPLC-quadrupole-Orbitrap mass spectrometry (UPLC-Q-Orbitrap MS). This method was validated by LODs, LOQs, precision, repeatability, stability, mean recovery, recovery range and RSD. The confirmed method was applied to the analysis of phenolic compounds in LR. Finally, 18 phenolic compounds in LR were qualitatively and quantitatively analyzed. Among them, 11 constituents were detected for the first time, which included two flavonoids (catechin and naringenin) and seven phenolic acids (gallic acid, vanillic acid, 2,4-dihydroxybenzoic acid, veratronic acid, benzoic acid, ellagic acid and salicylic acid). Moreover, Phloretin and protocatechuate, belonging to the dihydrochalcone flavonoid and protocatechuic acid respectively, were also identified and quantified. The total phenolics content (20.17 +/- 2.82 mg/g) and the total anthocyanin content (147.43 +/- 1.81 mg/g) were determined. In addition, the antioxidant activities of the LR extract were evaluated through 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP) and total antioxidant activity (T-AOC) assays.
关键词	Lycium Ruthenicum Murray Orbitrap Ms Phenolic Compounds Antioxidant
WOS标题词	Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
DOI	10.1016/j.foodchem.2018.06.132
关键词[WOS]	SIGNALING PATHWAY ; OXIDATIVE STRESS ; CHLOROGENIC ACID ; INFLAMMATION ; FLAVONOIDS ; RUTIN ; NARINGENIN ; PHLORETIN ; QUERCETIN ; GROWTH
收录类别	SCI
语种	英语
项目资助者	Qinghai Provincial Science Foundation(2016-ZJ-928Q ; CAS, China "Light of West China" Program (2016): Study on the effect of Lycium ruthenicum Murr. anthocyanin on the liver protection, Capacity Building Project of Engineering Research Center of Qinghai Province(2017-GX-G03) ; Innovation Platform for the Development and Construction of Special Project of Key Laboratory of Tibetan Medicine Research of Qinghai Province(2017-ZJ-Y11) ; 2015-NK-509 ; 2015-SF-120)
WOS研究方向	Chemistry ; Food Science & Technology ; Nutrition & Dietetics
WOS类目	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS记录号	WOS:000441142100019
出版者	ELSEVIER SCI LTD
引用统计	被引频次：53[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/13781
专题	中国科学院西北高原生物研究所
通讯作者	Hu, Na; Suo, Yourui
作者单位	1.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 2.Yanan Univ, Affiliated Hosp, Yanan 716000, Shaanxi, Peoples R China 3.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Qinghai, Peoples R China 4.Qinghai Prov Key Lab Tibetan Med Res, Xining 810001, Qinghai, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Gong,Chen, Shasha,Zhou, Wu,et al. Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity[J]. FOOD CHEMISTRY,2018,269:150-156.
APA	Zhang, Gong.,Chen, Shasha.,Zhou, Wu.,Meng, Jing.,Deng, Kai.,...&Suo, Yourui.(2018).Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity.FOOD CHEMISTRY,269,150-156.
MLA	Zhang, Gong,et al."Rapid qualitative and quantitative analyses of eighteen phenolic compounds from Lycium ruthenicum Murray by UPLC-Q-Orbitrap MS and their antioxidant activity".FOOD CHEMISTRY 269(2018):150-156.